摘要
Environmental pollution and the spread of pathogenic microorganisms pose a significant threat to the health of humans and the planet.Thus,understanding and detecting microorganisms is crucial for maintaining a healthy living environment.Nanopore sequencing is a single-molecule detection method developed in the 1990s that has revolutionized various research fields.It offers several advantages over traditional sequencing methods,including low cost,label-free,time-saving detection speed,long sequencing reading,real-time monitoring,convenient carrying,and other significant advantages.In this review,we summarize the technical principles and characteristics of nanopore sequencing and discuss its applications in amplicon sequencing,metagenome sequencing,and whole-genome sequencing of environmental microorganisms,as well as its in situ application under some special circumstances.We also analyze the advantages and challenges of nanopore sequencing in microbiology research.Overall,nanopore sequencing has the potential to greatly enhance the detection and understanding of microorganisms in environmental research,but further developments are needed to overcome the current challenges.
基金
grateful to the financial support from the National Natural Science Foundation of China(Nos.22025407,21974144)
Institute of Chemistry,Chinese Academy of Sciences。
作者简介
Ming-Qian Zhang(left)is currently a Ph.D.student at the Institute of Chemistry,Chinese Academy of Sciences(with Professor Hai-Chen Wu).Her research interest is the fabrication of new nanopores and their applications in biosensing;Xiao-Bin Huang(middle)is currently a Ph.D.student at the Institute of Chemistry,Chinese Academy of Sciences(with Professor Hai-Chen Wu).His research interest is applying nanopore techniques for in-situ singlecell analysis;Hai-Chen Wu(right)obtained his Ph.D.in organic chemistry under the supervision of Professors Jin-Quan Yu and Jonathan Spencer from University of Cambridge(Cambridge,UK)in 2005.He then carried out his postdoctoral research in the group of Professor Hagan Bayley in the Department of Chemistry,University of Oxford until the end of 2008.From 2009-2016,he was a principal investigator at the Key Laboratory for Biomedical Effects of Nanomaterials&Nanosafety,Institute of High Energy Physics,Chinese Academy of Sciences(Beijing,China).Since early 2017,he has been a principal investigator at the Key Laboratory for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences(Beijing,China).His research focuses on the development of new nanopore sensors and protein sequencing studies,E-mail:haichenwu@iccas.ac.cn。
