摘要
【目的】探讨酚酸类自毒物质对沙芥属植物生长及光合特性的影响,为沙芥属植物的野生资源保护和开发利用提供理论依据。【方法】以沙芥和斧翅沙芥幼苗为试验材料,选择肉桂酸和邻苯二甲酸2种自毒物质,采用盆栽试验,设置0,0.01,0.1,1,10 mmol/L浓度梯度处理,考察幼苗生长、叶绿素含量、叶绿素荧光参数和气体交换参数的变化特征。【结果】(1)肉桂酸、邻苯二甲酸对沙芥幼苗生长均表现为低浓度促进高浓度抑制,而对斧翅沙芥生长则表现为抑制作用,且均在浓度为10 mmol/L时对生长抑制作用最显著。(2)在不同浓度肉桂酸和邻苯二甲酸处理后,沙芥和斧翅沙芥幼苗叶片的叶绿素a、叶绿素b、叶绿素总量均出现不同程度下降。(3)沙芥和斧翅沙芥叶片净光合速率、蒸腾速率、气孔导度在肉桂酸和邻苯二甲酸浓度为10 mmol/L显著低于对照,胞间CO_(2)浓度此时无显著变化,其光合速率降低主要由非气孔因素所致。(4)沙芥和斧翅沙芥的最大光化学效率(F_(v)/F_(m))、实际光化学效率(Ф_(PSⅡ))和光化学淬灭系数(qP)均在肉桂酸和邻苯二甲酸浓度为10 mmol/L显著低于对照,而此时非光化学淬灭系数(NPQ)则显著高于对照。【结论】高浓度邻苯二甲酸和肉桂酸抑制沙芥和斧翅沙芥幼苗的光合作用,降低其光合速率,导致它们叶片的PSⅡ反应中心活性和开放程度受损,进而影响其正常生长;肉桂酸和邻苯二甲酸对沙芥属植物光合特性的影响存在差异,斧翅沙芥幼苗受到影响更大。
[Objective]In order to explore the effects of phenolic acid self-toxic substances on the growth and photosynthetic characteristics of Pugionium cornutum(L.)Gaertn.and P.dolabratum Maxim.,and to provide a theoretical basis for the protection,development,and utilization of wild resources of P.cornutum and P.dolabratum.[Methods]A pot experiment was conducted with P.cornutum and P.dolabratum seedlings.The experiment focused on two self-toxic substances,cinnamic acid and phthalic acid,and the seedlings were treated with varying concentrations of 0,0.01,0.1,1,and 10 mmol/L.The aim was to observe the effects of these concentrations on seedling growth,chlorophyll content,chlorophyll fluorescence parameters,and gas exchange parameters.[Results](1)Cinnamic acid and phthalic acid promoted seedling growth of P.cornatum at low concentrations and inhibited seedling growth at high concentrations.The growth of P.dolabratum was inhibited.10 mmol/L cinnamic acid and phthalic acid treatment had the most significant growth inhibition effect on Pugionium Gaertn.(2)Chl a,Chl b,and Chl a+b content of these two species were decreased in different degrees after treatment with different concentrations of cinnamic acid and phthalic acid.(3)Pn,Tr,and Gs of these two species at 10 mmol/L were significantly lower than that of the control,while Ci had no significant change.The main cause for the reduction of photosynthetic rate was non-stomatal factors.(4)F_(v)/F_(m),Ф_(PSⅡ),and qP of these two species at 10 mmol/L were significantly lower than that of the control and NPQ was significantly higher than control.[Conclusion]The high concentrations of phthalic acid and cinnamic acid inhibited photosynthesis and reduced photosynthetic rate in seedlings,resulting in the impairment of PSⅡreaction center activity and opening in leaves of these two species,which affected the growth of Pugionium Gaertn..In addition,the effects of cinnamic acid and phthalic acid on photosynthetic characteristics of Pugionium Gaertn.were different,due to different plant and stress types.
作者
宁可真
袁学松
杨忠仁
黄修梅
郝丽珍
张凤兰
NING Kezhen;YUAN Xuesong;YANG Zhongren;HUANG Xiumei;HAO Lizhen;ZHANG Fenglan(Inner Mongolia Key Laboratory of Wild Peculiar Vegetables Germplasm Resources and Germplasm Enhancement,College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010011,China;Inner Mongolia Autonomous Region Key Laboratory of Big Data Research and Application for Agriculture and Animal Husbandry,Hohhot 010011,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2024年第2期173-181,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金项目(31760570)
内蒙古自治区留学回国人员科技活动项目(启动类)
内蒙古自治区科技计划项目(2021GG0084)
内蒙古自治区应用技术研究与开发资金项目(2019GC237)
内蒙古自治区科技成果转化专项资金项目(2021CG0023)。
关键词
沙芥
斧翅沙芥
肉桂酸
邻苯二甲酸
光合特性
Pugionium cornutum(L.)Gaertn.
Pugionium dolabratum Maxim.
cinnamic acid
phthalic acid
photosynthetic characteristics
作者简介
宁可真(1995-),女,在读博士,主要从事蔬菜种质资源保存与创新研究。E-mail:2283913905@qq.com;通信作者:张凤兰,副教授,硕士导师,主要从事蔬菜种质资源保存与创新研究。E-mail:zhangfenglan041105@163.com。
