摘要
利用超声法提取黄精总黄酮,选取乙醇体积分数(A)、料液比(B)、超声时间(C)、超声温度(D)进行单因素试验,在单因素试验基础上,运用Box-Behnken试验设计,优化提取工艺;应用DPPH、ABTS及还原力测定等分析各组分抗氧化能力。结果表明:黄精总黄酮的最优提取工艺参数:料液比为1∶48.42 mL·g^(-1),提取时间50.00 min,乙醇浓度53.33%,温度54.66℃,在此条件下黄精总黄酮的提取率为30.12±0.03 mg·g^(-1),接近预测值,表明该响应面模型预测性较好;黄精总黄酮对DPPH和ABTS自由基的清除能力在0.25~3.00 mg·mL^(-1)范围内呈递增趋势,具有剂量依赖性,且在质量浓度为2~3 mg·mL^(-1)时,清除ABTS自由基能力与维生素C效果相当,显示其抗氧化活性较好,可作为良好的天然抗氧化剂。
Ultrasonic method was used to extract the total flavonoids of P.sibiricum.Single factor experiments were conducted with ethanol volume fraction(A),material liquid ratio(B),ultrasonic time(C),and ultrasonic temperature(D).On the basis of it,Box Behnken Design of experiments was used to optimize the extraction process with the total flavonoids extraction rate as the response value;Analyze the antioxidant capacity of each component using DPPH,ABTS,and reducing power measurements.The results showed that the optimal extraction process parameters for total flavonoids in P.sibiricum were as follows:the solid-liquid ratio was 1∶48.42 mL·g^(-1),the extraction time was 50.00 minutes,the ethanol concentration was 53.33%,and the temperature was 54.66℃.Under these conditions,the extraction rate of total flavonoids was 30.12±0.03 mg·g^(-1),which was close to the predicted value,indicating that the response surface model had good predictive ability;The scavenging ability of total flavonoids from P.sibiricum to DPPH and ABTS free radicals shows an increasing trend within a certain range,with a dose-dependent effect.At mass concentrations of 2 mg·mL^(-1)and 3 mg·mL^(-1),the scavenging ability of ABTS free radicals is equivalent to that of vitamin C.The total flavonoids from P.sibiricum also have good reducing power,indicating good antioxidant activity and can be used as a good natural antioxidant.
作者
张佳鑫
梁思琪
肖强
崔令军
Zhang Jiaxin;Liang Siqi;Xiao Qiang;Cui Lingjun(Key Laboratory of Biological Resources Protection and Utilization of Hubei Province Hubei Minzu University,Enshi 445000)
出处
《湖北林业科技》
2024年第1期37-44,共8页
Hubei Forestry Science and Technology
基金
湖北省自然科学基金创新发展联合基金项目“黄精黄酮合成分子机制解析与优质种质资源创制”(2023AFD077)
湖北省技术创新专项(重大项目)“道地中药材恩施黄精品种选育与富硒栽培及产品加工关键技术研发”(2019ACA120)
湖北民族大学博士启动基金项目“鄂西南珍贵用材楠属植物高效培育技术研究”(MY2017B029)。
关键词
黄精
总黄酮
超声提取
抗氧化活性
Polygonatum sibiricum
total flavonoids
ultrasonic extraction
antioxidant activity
作者简介
张佳鑫(1998-),女,硕士研究生在读,研究方向为天然产物活性成分分析与利用;通讯作者:崔令军。
