摘要
微流控芯片用于聚合酶链式反应(PCR)可提高检测效率和自动化程度,但目前把核酸提取、扩增、检测功能集成到一块芯片上仍比较困难,此外,微流控芯片专用PCR仪也存在温度控制不够快速、精准的问题。作者采用空间上温度循环代替时间上温度循环的设计思路,搭建了一套旋转式三温区微流控PCR扩增平台,对大肠杆菌进行核酸提取和扩增。结果表明,旋转式三温区微流控PCR扩增平台拥有较好的热均匀性和热稳定性,平台的升降温速率分别为3.5℃/s和2.67℃/s,单次循环时间为110 s。与9700型PCR仪相比,所建平台的温度控制方案更为简单、升降温速率更高、循环时间更短。本研究结果可为实现核酸提取、扩增一体化的微流控PCR设备的研发提供参考。
The use of microfluidic chips for polymerase chain reaction(PCR)can improve detection efficiency and automation.However,it is still difficult to integrate the nucleic acid extraction,amplification and detection functions into one chip.In addition,the temperature control of the special PCR instrument for microfluidic chip is not fast and accurate enough.Based on the principle of temperature cycling in space instead of temperature cycling in time,a rotary three-temperature zone microfluidic PCR amplification platform was built by the authors to extract and amplify E.coli nucleic acids.The results show that the rotary three-temperature zone microfluidic PCR amplification platform has good thermal uniformity and stability.The heating and cooling rates of the platform were 3.5℃/s and 2.67℃/s,respectively.The single cycle time is only 110 s.Compared with the 9700 PCR,the platform has a simpler temperature control scheme,higher heating and cooling rates and shorter cycle time.This study provides a reference for the development of microfluidic PCR devices that integrates nucleic acid extraction and amplification.
作者
孔振翔
姚延禄
周新丽
KONG Zhenxiang;YAO Yanlu;ZHOU Xinli(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2024年第2期107-112,共6页
Journal of Food Science and Biotechnology
关键词
微流控技术
聚合酶链式反应(PCR)
核酸扩增
microfluidic technology
polymerase chain reaction(PCR)
nucleic acid amplification
作者简介
通信作者:周新丽(1979-),女,博士,教授,博士研究生导师,主要从事微流控芯片研究。E-mail:zjulily@163.com。
