摘要
Terpenoids are the largest family of natural products.They are made from the building block isoprene pyrophosphate(IPP),and their bioproduction using engineered cell factories has received a great deal of attention.To date,the insufficient metabolic supply of IPP remains a great challenge for the efficient synthesis of terpenoids.In this work,we discover that the imbalanced metabolic flux distribution between the central metabolism and the IPP supply hinders IPP accumulation in Bacillus subtilis(B.subtilis).Therefore,we remodel the IPP metabolism using a series of genetically encoded two-input-multioutput(TIMO)circuits that are responsive to pyruvate or/and malonyl-CoA,resulting in an IPP pool that is significantly increased by up to four-fold.As a proof-of-concept validation,we design an IPP metabolism remodeling strategy to improve the production of three valuable terpenoids,including menaquinone-7(MK-7,4.1-fold),lycopene(9-fold),andβ-carotene(0.9-fold).In particular,the titer of MK-7 in a 50-L bioreactor reached 1549.6 mg·L^(-1),representing the highest titer reported so far.Thus,we propose a TIMO genetic circuits-assisted IPP metabolism remodeling framework that can be generally used for the synergistic fine-tuning of complicated metabolic modules to achieve the efficient bioproduction of terpenoids.
基金
financially supported by the National Natural Science Foundation of China(32070085,32200050,31871784,and 32021005)
the Natural Science Foundation of Jiangsu Province(BK20221079)
National Postdoctoral Program for Innovative Talents(BX20220136)
the Jiangsu Funding Program for Excellent Postdoctoral Talent(2022ZB498)
the Fundamental Research Funds for the Central Universities(JUSRP52019A,JUSRP121010 and JUSRP221013)
National Key Research and Development Program of China(2020YFA0908300)
Postgraduate Research&Practice Innovation Program of Jiangsu Provence(KYCX18_1797)。
作者简介
Corresponding authors:Jian Chen.E-mail addresses:jchen@jiangnan.edu.cn;Corresponding authors:Long Liu,E-mail addresses:longliu@jiangnan.edu.cn。
