摘要
核苷类药物在新型冠状病毒肺炎(COVID-19)、艾滋病及肝炎等病毒性疾病治疗方面效果显著,应用广泛,其准确定量具有重要意义。近年来,基于分子印迹技术合成的分子印迹聚合物(MIPs)备受关注。MIPs可模拟酶与底物或抗体与抗原之间的相互作用,对目标物进行高选择性识别。将MIPs应用于核苷类药物分离分析过程,可显著提高选择性和检测灵敏度。本文对近5年MIPs在核苷类药物样品前处理(固相萃取、固相微萃取等)、色谱分离及传感分析等检测中的应用进行综述,总结了检测过程中面临的挑战,并对其应用前景进行了展望。
Nucleoside drugs are effective in the treatment of novel coronavirus pneumonia(COVID-19),HIV,hepatitis and other viral diseases in recent years.Therefore,the accurate quantitative analysis of them is of great significance.For the past few years,molecularly imprinted polymers(MIPs) synthesized by molecular imprinting technology have attractedmuch attention.The MIPs can stimulate the interactions between enzymes with substrates or antibodies and antigens,and support a recognition of the target with high selectivity.The selectivity and sensitivity can be greatly improved using MIPs in the separation and analysis of nucleoside drugs.In this review,the applications of MIPs in three fields including sample pretreatment(solid phase extraction,solid phase microextraction,etc.),chromatographic separation and sensor analysis of nucleoside drugs in the last five years are summarized.The advantages of MIPs in detection of nucleoside drugs are discussed.Meanwhile,the challenges and prospects are expected.
作者
薛俊萍
魏国
柴佩君
吴文璞
张异梦
李桂生
宋志花
XUE Junping;WEI Guo;CHAI Peijun;WU Wenpu;ZHANG Yimeng;LI Guisheng;SONG Zhihua(Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation, School of Pharmacy, Yantai University)
出处
《分析试验室》
EI
CAS
CSCD
北大核心
2024年第1期118-127,共10页
Chinese Journal of Analysis Laboratory
基金
烟台市科技发展计划(2020MSGY112)
药学国家级实验教学示范中心(烟台大学)项目资助。
关键词
分子印迹聚合物
核苷类药物
样品前处理
色谱分析
传感分析
molecularly imprinted polymers(MIPs)
nucleoside drugs
sample pretreatment
chromatographic analysis
sensor analysis
作者简介
薛俊萍,女(1998-),硕士研究生。研究方向:工业药学,xjp19980810@163.com;通信作者:李桂生,男(1967-),博士,副教授。研究方向:生药学,guisheng@luyetz.com;通信作者:宋志花,女(1987-),博士,副教授。研究方向:药物分析,zhihuasong08@yeah.net。
