摘要
Viral envelope fusion with the host plasma membrane(PM)for genome release is a hallmark step in the life cycle of many enveloped viruses.This process is regulated by a complex network of biomolecules on the PM,but robust tools to precisely elucidate the dynamic mechanisms of virus-PM fusion events are still lacking.Here,we developed a quantitative single-virus tracking approach based on highly efficient dual-color labelling of viruses and batch trajectory analysis to achieve the spatiotemporal quantification of fusion events.This approach allows us to comprehensively analyze the membrane fusion mechanism utilized by pseudotyped severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)at the singlevirus level and precisely elucidate how the relevant biomolecules synergistically regulate the fusion process.Our results revealed that SARS-CoV-2 may promote the formation of supersaturated clusters of cholesterol to facilitate the initiation of the membrane fusion process and accelerate the viral genome release.
作者
刘昊阳
胡宇斯
余聪
王志刚
刘书琳
庞代文
Hao-Yang Liu;Yusi Hu;Cong Yu;Zhi-Gang Wang;Shu-Lin Liu;Dai-Wen Pang(State Key Laboratory of Medicinal Chemical Biology,Frontiers Science Center for New Organic Matter,Frontiers Science Center for Cell Responses,Tianjin Key Laboratory of Biosensing and Molecular Recognition,Research Center for Analytical Sciences,College of Chemistry,and School of Medicine,Nankai University,Tianjin 300071,China;Haihe Laboratory of Sustainable Chemical Transformations,Tianjin 300192,China)
基金
supported by the National Natural Science Foundation of China(22293032,22293030,and 91859123)
the National Key Research and Development Program of China(2019YFA0210500)
the Fundamental Research Funds for the Central Universities of China(63211023)
the financial support from Haihe Laboratory of Sustainable Chemical Transformations.
作者简介
Hao-Yang Liu received his B.S.degree(2012)from Henan University of Technology,and M.S.degree(2015)from Wuhan University.In 2022,he completed his doctoral studies at the College of Chemistry,Nankai University.Currently,he holds a postdoctoral position at the University of Texas at Austin.His research primarily revolves around drug delivery in vivo and the development of fluorescence imaging techniques for various bioapplications;Corresponding authors:Shu-Lin Liu obtained her B.S.degree from Zhengzhou University(2007)and Ph.D.degree(2013)at College of Chemistry and Molecular Sciences,Wuhan University.She worked as a postdoctoral researcher at University of Illinois at Chicago(2013-2017).After that,she worked as a professor at College of Chemistry,Nankai university.Her research mainly focuses on the establishment of in situ quantitative methods for monitoring viral infection progression,and the design and application of virus-mimicking nanosystems.E-mail addresses:shulin.liu@nankai.edu.cn;Corresponding authors:Dai-Wen Pang graduated with his B.S.degree in Chemistry from Wuhan University in 1982,and received his Ph.D.degree from Wuhan University in 1992.He is now a distinguished professor at Nankai University.His research interest focuses on biomedical used quantum dots(QDs),including space-time coupled living-cell synthesis of QDs,quasi-bio synthesis of QDs,single-virus tracking with QDs,photoluminescence mechanism of luminescent nanomaterials,and backlight display with QDs.E-mail addresses:dwpang@whu.edu.cn。
