摘要
【目的】探讨麻竹扦插生根过程及其相关生理生化变化,为麻竹扦插繁殖提供理论基础和实践指导。【方法】以麻竹主枝为试验材料,采用生长调节剂吲哚丁酸(IBA)和萘乙酸(NAA)按1∶1配比对插穗进行浸泡处理,其中,IBA和NAA分别设置50、100、200、300 mg/L 4个浓度水平,以清水为对照组,对其根系发生、生根效果及生根过程中内源激素、氧化酶活性和营养物质含量进行测定。【结果】(1)以200 mg/L IBA+200 mg/L NAA处理麻竹主枝的生根效果最佳,极显著优于对照组及其他处理组;(2)根据麻竹主枝扦插生根过程形态变化,可将其生根过程分为3个阶段:0~20 d为根系诱导阶段,21~60 d为根系伸长阶段,60 d后为根系成熟阶段;结合根系内部解剖结构看,麻竹扦插生根类型属于潜伏不定根原基生根型。(3)在生根过程中,处理组IAA含量高于对照组,均呈先上升后下降的趋势;处理组ABA含量的变化趋势在根系成熟期前上升,后逐渐下降,对照组则在40 d前下降,40 d后逐渐上升;处理组CTK含量在40 d时开始下降,成熟期后逐渐上升,对照组CTK含量在伸长期下降,40 d后逐渐上升;处理组GA_(3)含量在20~40 d下降,在40 d后逐渐上升,而对照组GA_(3)含量在成熟期前一直处于下降状态。(4)处理组和对照组SOD活性在根系诱导期最高,随后逐渐下降,80 d后回升;POD活性在诱导期后有所上升,40 d时逐渐下降,根系成熟期时趋于稳定,对照组的POD活性始终处于较低水平;IAAO活性从根系诱导期到伸长期逐渐上升,40 d后缓慢下降,处理组的IAAO活性高于对照组;处理组和对照组的PPO活性在根系成熟期前均呈先上升后下降的趋势,成熟期后逐渐下降。(5)可溶性糖和可溶性蛋白含量在扦插过程中的变化均为在根系成熟期前逐渐下降,根系成熟期后逐渐上升。【结论】IBA+NAA处理对麻竹扦插生根的促进作用显著优于对照组,生根过程中内源激素、氧化酶活性和营养物质的共同协调影响着麻竹扦插不定根的形成和生长,为麻竹扦插繁殖提供了理论基础和实践指导。
【Objective】To explore the rooting process of Dendrocalamus latiflorus cuttings and its related physiological changes,and to provide a theoretical basis and practical guidance for the research of D.latiflorus cuttings propagation technology.【Method】The cuttings were treated with the growth regulator IBA and NAA in a 1:1 ratio at four concentration levels of 50,100,200 and 300 mg/L,respectively,with water as the control.The roots,rooting effect,rooting process and changes in endogenous hormones,oxidase activity and nutrients during the rooting process were measured to investigate the types of rooting and the physiological basis of rooting in D.latiflorus cuttings.【Result】(1)The rooting effect of 200 mg/L IBA+NAA treatment on D.latiflorus branches was the best and significantly better than that of the control group;(2)To observe the morphological changes of the rooting process of the main branch of D.latiflorus bamboo,the rooting process could be divided into three stages:0-20 d for the root induction stage,21-60 d for the root elongation stage,and 60 d for the root maturation stage.Combined with the internal anatomical structure of the root system,the type of rooting of D.latiflorus cuttings belong to the submerged adventitious root primordial rooting type.(3)During the rooting process,the IAA content of the treated group was higher than that of the control group,both showing an increasing trend followed by a decreasing trend.The trend of ABA content in the treatment group increased before root maturity and then gradually decreased,while in the control group it decreased before 40 d and gradually increased after 40 d.The CTK content of the treated group started to decrease at 40 d and gradually increased after maturity,while the CTK content of the control group decreased during the elongation period and gradually increased after 40 d.The GA_(3)content of the treatment group decreased from 20-40 d and gradually increased after 40 d,while the GA_(3)content of the control group remained in a declining state until maturity.(4)SOD activity in the treated and control groups was highest at the root induction stage,then gradually declined and rose again after 80 d.POD activity increased after the induction stage,then gradually declined at 40 d,and stabilized at the root maturity stage,while POD activity in the control group was always at a lower level;IAAO activity gradually increased from root induction to elongation stage then slowly declined after 40 d.IAAO activity in the treated group was higher than that in the control group;PPO activity in both the treated and control groups tended to increase before the root maturity stage,then gradually declined after the maturity stage.(5)Both soluble sugars and soluble protein content changed during the cuttings,gradually decreasing before the root maturity stage and gradually increasing after the root maturity stage.【Conclusion】The IBA+NAA treatment has a significantly better effect on rooting of D.latiflorus cuttings than the control group.The joint coordination of endogenous hormones,oxidase activity and nutrients in the rooting process affects the formation and growth of adventitious roots of D.latiflorus cuttings,providing a theoretical basis and practical guidance for the study of D.latiflorus cutting propagation technology.
作者
黄锈贤
郭松
贾冬冬
徐振国
李在留
HUANG Xiu-xian;GUO Song;JIA Dong-dong;XU Zhen-guo;LI Zai-liu(College of Forestry,Guangxi University,Nanning 530004,Guangxi,China;Guangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation,Guangxi University,Nanning 530004,Guangxi,China;Key Laboratory of National Forestry and Grassland Administration on Cultivation of Fast-Growing Timber in Central South China,Guangxi University,Nanning 530004,Guangxi,China;Guangxi Forestry Research Institute,Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation,Nanning 530002,Guangxi,China;College of Architecture and Arts,Guangxi Arts University Academy,Nanning,530007,Guangxi,China)
出处
《经济林研究》
北大核心
2023年第4期245-253,共9页
Non-wood Forest Research
基金
中央财政林业科技推广示范项目([2021]TG 20号)
国家自然科学基金项目(31560200)。
关键词
麻竹
主枝扦插
根系发生
内源激素
营养物质
氧化酶活性
Dendrocalamus latiflorus
main branch cuttings
root system occurs
endogenous hormones
nutrients
oxidase activity
作者简介
第一作者:黄锈贤(18078728408@163.com),硕士;通信作者:李在留(lizailiu666@163.com),博士,副教授;通信作者:徐振国(xuopingqing@126.com),硕士,高级工程师。
