摘要
目的 考察双歧杆菌四联活菌中4种益生菌在模拟消化液中的耐受性。方法 按照现行版《中国药典》配制人工胃液与人工肠液,将4种益生菌分别加入其中,充分混匀,孵育0、15、30、60、90、120、180 min后,稀释、涂布与计数,绘制每种菌存活的衰减曲线,考察其存活率。结果 在人工胃液(pH2.5~4.5)孵育3 h后,长双歧杆菌婴儿亚种(GMCC 0460.1)、嗜酸乳杆菌(GMCC 0460.2)、粪肠球菌(GMCC0460.3)、蜡样芽胞杆菌(GMCC0460.4)的存活率分别为53.23%~61.30%、 75.31%~105.34%、90.76%~100.00%、81.02%~102.58%;在人工肠液(胆汁盐浓度0.03%~0.20%)孵育3 h后,长双歧杆菌婴儿亚种、嗜酸乳杆菌、粪肠球菌、蜡样芽胞杆菌的存活率分别为42.19%~71.49%、 16.62%~45.03%、40.80%~69.50%、42.67%~95.43%。结论 4种益生菌在较长时间内耐受人工胃液(pH 2.5~4.5)和人工肠液(胆汁盐浓度0.03%~0.20%),菌体存活量下降不到1个数量级,对胃酸和胆盐的耐受性较强。由于健康人体内胃肠的环境类似于上述环境,预示其在健康人体消化道内能较好保持菌体生存能力,发挥药效活性。
Objective To observe the tolerance of bifidobacterium-quadruple probiotics in simulated digestive fluid. Methods The simulated gastric fluid and simulated intestinal fluid were prepared according to the current version of the Chinese pharmacopoeia. Four kinds of probiotics were separately added in the simulated fluids, thoroughly mixed, and incubated for 0 min, 15 min, 30 min, 60 min, 90 min, 120 min and 180 min respectively, then diluted, coated on the plates and counted. The survival attenuation curve of each bacterium was plotted and its survival rate was observed. Results After incubation in the simulated gastric fluid (pH 2.5 - 4.5) for 3 hours, the survival rates of Bifidobacterium longum subsp. infantis (GMCC 0460.1), Lactobacillus acidophilus (GMCC 0460.2), Enterococcus faecalis (GMCC 0460.3) and Bacillus cereus (GMCC 0460.4) were 53.23% - 61.30%, 75.31% - 105.34%, 90.76% - 100.00% and 81.02% - 102.58%, respectively. Likely, the survival rates of Bifidobacterium longum subsp. infantis, Lactobacillus acidophilus, Enterococcus faecalis and Bacillus cereus in the simulated intestinal fluid (bile salt concentration 0.03% - 0.20%) were 42.19% - 71.49%, 16.62% - 45.03%, 40.80% - 69.50% and 42.67% - 95.43%, respectively. Conclusion The four probiotics showed strong tolerance under the conditions of incubation with simulated gastric fluid (pH 2.5 - 4.5) and simulated intestinal fluid (bile salt concentration 0.03% - 0.20%), with the survival numbers of them decreased less than one order of magnitude. The gastrointestinal environment of healthy people is similar to the simulated intestinal fluid, indicating that the four probiotics have strong tolerance to gastric acid and bile salts, which can effectively guarantee their pharmacological activities.
作者
王露霏
王彩云
李嘉文
章朦玥
张璐
张怡轩
WANG Lufei;WANG Caiyun;LI Jiawen;ZHANG Mengyue;ZHANG Lu;ZHANG Yixuan(School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang,Liaoning 110016,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
2023年第11期1280-1285,1291,共7页
Chinese Journal of Microecology
关键词
双歧杆菌
益生菌
人工胃液
人工肠液
耐受性
Bifidobacterium
Probiotics
Simulated gastric fluid
Simulated intestinal fluid
Tolerance
作者简介
王露霏(1998-),女,硕士,微生物与生化药学专业,E-mail:951548324@qq.com;通信作者:张怡轩,女,教授,博士生导师,从事生物技术制药教学与科研,E-mail:zhangyxzsh@163.com。
