摘要
为研究南极磷虾磷脂的组成及抗氧化活性,采用硅胶柱层析法分离制备南极磷虾磷脂,对其磷脂组成和磷脂脂肪酸组成进行分析,通过测定自由基清除能力和还原能力系统评价南极磷虾磷脂的抗氧化活性。结果表明,纯化获得的南极磷虾磷脂的磷脂含量为(93.78±3.18)g/100 g;南极磷虾磷脂主要为磷脂酰胆碱和磷脂酰乙醇胺,分别占比(90.60±1.03)%和(5.08±1.10)%;南极磷虾磷脂中多不饱和脂肪酸含量丰富,且主要为二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahexaenoic acid,DHA),分别占总脂肪酸含量的(31.75±0.24)%和(19.35±0.10)%。南极磷虾磷脂具有良好的自由基清除能力和还原能力,其清除ABTS+自由基和DPPH自由基的IC_(50)值分别为15.78 mg/mL和14.87 mg/mL,且其还原能力呈浓度依赖效应。南极磷虾磷脂的抗氧化能力明显优于大豆磷脂和卵黄磷脂。
The composition and antioxidant activity of phospholipids from Antarctic krill(Euphausia superba)were investigated in this study.The phospholipids from Antarctic krill were separated and prepared by silica gel column chromatography,and then the composition and fatty acid composition of the obtained phospholip-ids were analyzed.The antioxidant activity of the obtained phospholipids was systematically evaluated by mea-suring the free radical scavenging capacity and reducing capacity.The results showed that the purity of the ob-tained phospholipids was(93.78±3.18)g/100 g.The phospholipids from Antarctic krill were mainly composed of phosphatidylcholine and phosphatidylethanolamine,accounting for(90.60±1.03)%and(5.08±1.10)%of the total phospholipids,respectively.The phospholipids from Antarctic krill were rich in polyunsaturated fatty acids,mainly eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA),accounting for(31.75±0.24)%and(19.35±0.10)%of the total fatty acids,respectively.The phospholipids from Antarctic krill exhibited good free radical scavenging capacity and reducing capacity.The IC_(50) of scavenged ABTS+free radical and DPPH free radical was 15.78 mg/mL and 14.87 mg/mL,respectively,and the reducing capacity presented a concentration-dependent effect.The antioxidant activity of phospholipids from Antarctic krill was obviously su-perior to that of soybean phospholipids and yolk phospholipids.
作者
刘小芳
郭向融
李爽
辛云
蒋永毅
冷凯良
姜维
LIU Xiaofang;GUO Xiangrong;LI Shuang;XIN Yun;JIANG Yongyi;LENG Kailiang;JIANG Wei(Key Laboratory of Sustainable Development of Polar Fisheries,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,Shandong,China;National Engineering Research Center for Marine Aquaculture,Zhejiang Ocean University,Zhoushan 316022,Zhejiang,China;Qingdao Future Testing Technology Co.,Ltd.,Qingdao 266112,Shandong,China)
出处
《食品研究与开发》
CAS
2024年第3期16-21,共6页
Food Research and Development
基金
国家自然科学基金青年科学基金项目(32001770)
国家重点研发计划项目(2022YFC2807505)。
关键词
南极磷虾
磷脂
纯化
组成特征
抗氧化活性
Antarctic krill(Euphausia superba)
phospholipids
purify
composition characteristics
anti⁃oxidant activity
作者简介
刘小芳(1987-),女(汉),副研究员,博士,研究方向:水产品加工与利用;通信作者:姜维(1988-),男(汉),研究员,博士,研究方向:水产品加工与利用。
