摘要
该文建立液相色谱-稳定同位素比值质谱联用(liquid chromatography-stable isotope ratio mass spectrometry,LC-IRMS)检测抗坏血酸δ^(13)C值的分析方法,用于鉴别针叶樱桃粉中抗坏血酸天然来源的真实性。样品中抗坏血酸经液相色谱在线分离纯化,优化后色谱条件为:Syncronis C18(250 mm×4.6 mm,5μm)色谱柱,流动相为水-pH 2的硫酸溶液(90∶10,体积比),流速0.250 mL/min,色谱柱温度30℃,进样量10μL,通过LC-IsoLink实现目标物全部氧化为CO_(2)气体,最终以气态形式进入稳定同位素质谱仪,直接检测样品中抗坏血酸的δ^(13)C,该方法结果稳定、准确。分别测定了7个合成来源的维生素C片和19个针叶樱桃粉,结果表明,天然来源抗坏血酸δ^(13)C值为-25.00‰~-22.01‰,合成来源抗坏血酸δ^(13)C值为-11.74‰~-10.28‰,两者分布显著性差异,该方法可用于抗坏血酸产品标识的真实性鉴别研究。
An analytical method for the detection of ascorbic acidδ^(13)C value by liquid chromatography-stable isotope ratio mass spectrometry(LC-IRMS)was established to identify the authenticity of the natural source of ascorbic acid in acerola cherry powder.Ascorbic acid in the sample was separated and purified by liquid chromatography,and the optimized chromatographic conditions were that Syncronis C18(250 mm×4.6 mm,5 mm)column was used,the mobile phase was the aqueous-sulfuric acid solution(90∶10,volume ratio),the flow rate was 0.250 mL/min,the column temperature was 30℃,the injection volume was 10 mL,and all the targets were oxidized to CO_(2)gas by LC-IsoLink,and finally entered the stable isotope mass spectrometer in gaseous form.Theδ^(13)C of ascorbic acid in the sample was directly detected,and the results of this method were stable and accurate.Results showed that the distribution range of natural ascorbic acidδ^(13)C value was-25.00‰--22.01‰,and the distribution of synthetic ascorbic acidδ^(13)C value was-11.74‰--10.28‰,which was significantly different.This method could be used for the authenticity identification of ascorbic acid product identification.
作者
王道兵
冯迪
曹翠峰
钟其顶
翟鹏贵
武竹英
岳红卫
童玲
张燚
洪玉玲
张红霞
徐胜
张洛琪
WANG Daobing;FENG Di;CAO Cuifeng;ZHONG Qiding;ZHAI Penggui;WU Zhuying;YUE Hongwei;TONG Ling;ZHANG Yan;HONG Yuling;ZHANG Hongxia;XU Sheng;ZHANG Luoqi(Technology Innovation Center of Light Industrial Consumer Goods Quality and Safety for State Market Regulation,Beijing 100015,China;Sinolight Technology Innovation Centre Co.Ltd.,Beijing 100015,China;China National Research Institute of Food and Fermentation Industries Co.Ltd.,Beijing 100015,China;Yangshengtang Pharmaceutical Co.Ltd.,Haikou 570216,China;Nongfu Spring Co.Ltd.,Hangzhou 310024,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第2期283-287,295,共6页
Food and Fermentation Industries
基金
国家重点研发计划(2022YFF0710403)
国家重点研发计划(2022YFF0606800)
北京市朝阳区科技计划(CYGX2104)。
作者简介
第一作者:王道兵,高级工程师;通信作者:钟其顶,教授级高级工程师,E-mail:zhongqiding@163.com。
