摘要
本研究从健康犊牛粪便中分离益生菌,并分析其益生特性。通过形态学观察和16S rDNA测序进行鉴定,对其抗菌活性和抑菌物质分析,建立生长曲线和产酸曲线,探究其黏附能力和耐受性,通过药敏试验和溶血试验评判其安全性。结果显示,4株益生菌为敏捷乳杆菌(Lactobacillus agilis)、戊糖片球菌(Pediococcus pentosaceus)、唾液乳杆菌(Lactobacillussalivarius)和约氏乳杆菌(Lactobacillusjohnsonii),且对大肠杆菌(Escherichia coli)O111:K58(B4)、金黄色葡萄球菌(Staphylococcus aureus)和大肠杆菌K99(Escherichia coliK99)均具有抑菌活性,其中12和30号菌株的抑菌物质为有机酸和过氧化氢,26号菌株还包括细菌素,14号菌株为有机酸和细菌素,其生长特性和产酸能力良好,具有较强的细胞黏附能力,对胆盐溶液和人工胃肠液的耐受性较强,对抗生素敏感性较强,无溶血性。本研究分离得到的4株益生菌符合寻找替抗物质的需求,为防治细菌性犊牛腹泻提供了理论依据。
In this study,probiotics were isolated from the feces of healthy calves and its probiotic properties were analyzed.The probiotics were identified by morphological observation and 16S rDNA sequencing followed by analysis of their antibacterial activity and antibacterial substances.The growth curve and acid production curve were established to explore their adhesion ability and tolerance.The safety was evaluated by drug sensitivity test and hemolysis test.The results showed that four probiotics were identified as Lactobacillus agilis,Pediococcus pentosaceus,Lactobacillus salivarius and Lactobacillus johnsonii that displayed antibacterial activities against Escherichia coli O111:K58(B4),Staphylococcus aureus and E.coli K99.The antibacterial substances of strain 12 and 30 were organic acids and hydrogen peroxide.Bacteriocins were found in strain 26 in addition to organic acids and hydrogen peroxide.Strain 14 produced organic acids and bacteriocin.They showed good growth characteristics,acid production ability, strong cell adhesion ability, strong tolerance to bile salt solution and artificial gastroentericfluid, strong sensitivity to antibiotics, and no hemolytic. The four probiotics strains isolated in this study met the needof searching for antifungal substances, and provided a theoretical basis for the prevention and treatment of bacterialdiarrhea in calves.
作者
冯鸣鹊
靳天雄
刘蓓
苏娅澜
仝静迪
赵岩
程佳
刘明超
FENG Mingque;JIN Tianxiong;LIU Bei;SU Yalan;TONG Jingdi;ZHAO Yan;CHENG Jia;LIU Mingchao(College of Veterinary Medicine,Hebei Agricultural University,Baoding 071001,China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2024年第1期91-98,131,共9页
Journal of Hebei Agricultural University
基金
国家自然科学基金项目(31902328)
河北省自然科学基金项目(C2021204067)
河北省省属高等学校基本科研业务费研究项目(KY2021008)
河北农业大学引进人才科研专项项目(ZD201723)。
关键词
犊牛
益生菌
抑菌活性
腹泻
calf
probiotics
antibacterial activity
diarrhea
作者简介
第一作者:冯鸣鹊(2000-),女,河北邯郸人,硕士研究生,从事临床兽医学研究.E-mail:1252356224@qq.com;通信作者:刘明超(1987-),男,山东青州人,博士,副教授,从事临床兽医学研究.E-mail:liumingchao@163.com;通信作者:程佳(1991-),女,山东潍坊人,博士,副教授,从事临床兽医学研究.E-mail:chengjia@hebau.edu.cn。
