摘要
荧光免疫层析技术是近年来开发的一种新型免疫检测技术,以荧光微球为标记物,既保留了传统胶体金试纸条检测速度快、价格便宜、操作简单、携带方便等优点,又通过荧光技术提高了检测的灵敏度。荧光免疫层析技术对实验设备、资源的要求低,特别适合在条件有限的实验室及野外开展动物疾病快速诊断。本文详细介绍了荧光免疫层析技术的原理及其在病毒、细菌、寄生虫等病原检测方面的应用研究,以期为病原微生物现场快速诊断技术的开发提供参考。
Fluorescence immunochromatography is a new immunodetection technology developed in recent years,which,taking fluorescent microsphere as a marker,not only retains the advantages of rapidity,low cost,simple operation and convenience,but also improves sensitivity with the help of fluorescence technology.The technology is particularly suitable for rapid diagnosis of animal diseases in laboratories with limited conditions and in the field,as no more experimental devices or resources are required.In the paper,the principle of the technology and its application in the detection of viruses,bacteria and parasites were introduced,with a view to providing a reference for on-site rapid detection of pathogenic microorganisms.
作者
苏华彬
刘蒙达
南文龙
孙明军
焉鑫
毛迎雪
曲瑶
孙淑芳
李桂梅
樊晓旭
Su Huabin;Liu Mengda;Nan Wenlong;Sun Mingjun;Yan Xin;Mao Yingxue;Qu Yao;Sun Shufang;Li Guimei;Fan Xiaoxu(China Animal Health and Epidemiology Center,Qingdao 266032,Shandong,China;College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,Shandong,China;Key Laboratory Ruminant Infectious Disease Prevention and Control(East China),Ministry of Agriculture and Rural Affairs,Qingdao 266032,Shandong,China;College of Animal Science,Shandong Agricultural University,Tai'an 271000,Shandong,China)
出处
《中国动物检疫》
CAS
2024年第1期67-71,共5页
China Animal Health Inspection
基金
“十四五”国家重点研发计划项目(2022YFD1800701)
国家奶牛产业技术体系资助项目(CARS36)。
关键词
荧光免疫层析技术
荧光微球
病原微生物检测
fluorescence immunochromatography
fluorescent microsphere
detection of pathogenic microorganisms
作者简介
同等贡献作者:苏华彬;同等贡献作者:刘蒙达;通信作者:孙淑芳。E-mail:sunshufang@cahec.cn;通信作者:李桂梅。E-mail:201201054@qau.edu.can;通信作者:樊晓旭。E-mail:fanxiaoxu@cahec.cn。
