摘要
为探究鰤鱼诺卡氏菌灭活疫苗对石斑鱼的免疫效果,通过免疫基因表达量分析疫苗对石斑鱼的免疫保护。利用鰤鱼诺卡氏菌制备福尔马林灭活全菌疫苗,通过腹腔注射法对珍珠龙胆石斑鱼进行免疫,利用荧光定量法检测免疫后该鱼的肾脏、肝脏和脾3个器官中TLR2、MyD88、TNF-α、IL-1β免疫基因表达量的变化。结果显示,注射灭活疫苗后,TNF-α、IL-1β基因表达量在肾脏组织中较高,MyD88基因表达量在脾组织中较高,TLR2基因表达量在肝脏组织中较高;除脾组织中的TNF-α基因相对表达量出现下降趋势外,其余基因的表达量在所有器官中都呈现出上调的趋势。研究结果为探索珍珠龙胆石斑鱼与诺卡氏菌的相互作用提供理论基础,为渔业疫苗开发和应用奠定基础。
In order to explore the immune effect of inactivated vaccine of Nocardia seriolae on grouper,the immune protection of the vaccine on grouper was analyzed by the expression of immune gene.In this study,formalininactivated vaccine was prepared by this bacteria,and the pearl gentian grouper was immunized by intraperitoneal injection.The expression changes of TLR2,MyD88,TNF-αand IL-1βimmune gene in the kidney,liver and spleen of the fish were detected by fluorescence quantitative method.The results showed that by injecting inactivated vaccine,the expressions of TNF-αand IL-1βgenes were higher in kidney tissues,MyD88 gene’s expression was higher in spleen tissues,and TLR2 gene’s expression was higher in liver tissues.Except for a trend of decreasing relative expression of TNF-αgene in the tissue spleen,and the other genes showed up-regulation in all organs compared with that in blank group.Above results provided a theoretical basis for exploring the interaction between pearl gentian grouper and Nocardia seriolae,and laid a foundation for the development and application of fishery vaccines.
作者
高浩峰
邵蓬
武尊
吴雅婷
汪笑宇
GAO Haofeng;SHAO Peng;WU Zun;WU Yating;WANG Xiaoyu(Tianjin Key Laboratory of Aqua-ecology and Aquaculture,College of Fisheries,Tianjin Agricultural University,Tianjin 300392,China;Tianjin Fisheries Research Institute,Tianjin 300392,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2024年第2期145-152,共8页
Journal of Agricultural Science and Technology
基金
天津市科技支撑项目(18YFZCNC01190)。
作者简介
高浩峰,E-mail:supermeiking@163.com;通信作者:邵蓬,E-mail:shaopeng@tjau.edu.cn。
