摘要
目的:观察电针预处理“百会”“大椎”对缺血性中风小鼠运动功能、脑皮层血流灌注、小胶质细胞和血清炎性因子白细胞介素1β(IL-1β)、转化生长因子β(TGF-β)、白细胞介素10(IL-10)含量的影响,探讨电针预处理改善缺血性中风后运动功能的机制。方法:C57BL/6小鼠随机分为假手术组、模型组、电针预处理组(电针组),每组15只。光栓诱导构建单侧缺血性卒中运动障碍模型。电针组小鼠在造模前进行连续7 d的电针“百会”“大椎”预处理,每次20 min,每天1次。用网格实验和爬杯实验评价小鼠造模前后运动功能;激光散斑血流监测视频系统评估小鼠初级运动皮层血流灌注量;ELISA法检测小鼠血清IL-1β、TGF-β、IL-10的含量;免疫荧光染色法检测小鼠初级运动皮层小胶质细胞及其M2亚型细胞标记物的表达。结果:造模后,与假手术组比较,模型组小鼠网格错误率、患肢拖拽率升高(P<0.01),患肢利用率及患侧占健侧皮层血流灌注量百分比降低(P<0.01);血清IL-1β、TGF-β、IL-10含量升高(P<0.01,P<0.05);患侧初级运动皮层小胶质细胞呈阿米巴样,离子钙接头蛋白分子1(IBA1)和CD206荧光强度升高(P<0.01)。与模型组比较,电针组小鼠网格错误率、患肢拖拽率降低(P<0.01),患肢利用率及患侧与健侧血流灌注量百分比升高(P<0.05);血清IL-1β含量降低(P<0.01),TGF-β、IL-10含量升高(P<0.01);患侧初级运动皮层小胶质细胞胞体较模型组更圆、更密集,IBA1和CD206荧光强度升高(P<0.01)。结论:电针预处理“百会“”大椎”可提高梗死局部小胶质细胞尤其是M2亚型标记物的表达,提高血清抗炎因子含量及降低促炎因子含量,从而减轻炎性反应。
ObjectiveTo investigate the effects on the m otor function,cortex blood flow perfusion,m icroglial cells,and the contents of serum inflam m atory factors,i.e.interleukin-1β(IL-1β),transform ing growth factor-β(TGF-β),and interleukin-10(IL-10)after electroacupuncture(EA)preconditioning at“Baihui”(GV20)and“Dazhui”(GV14)in the m ice with ischem ic stroke,so as to explore the m echanism of EA preconditioning for im proving m otor function after ischem ic stroke.MethodsC57BL/6 m ice were random ly divided into sham-operation group,m odel group,and EA preconditioning group(EA group),with 15 m ice in each group.A photothrom botic m ethod was used to induce the m odel of unilateral ischem ic stroke and m otor im pairm ent.The m ice in the EA group received EA preconditioning,20 m in each tim e,once daily for 7 consecutive days before m odeling.The m otor function of m ice was evaluated by the grid-walking test and cylinder test before and after m odeling.Laser speckle blood flow video m onitoring system was em⁃ployed to assess the cerebral blood flow perfusion in the prim ary m otor cortex of m ice.The contents of IL-1β,TGF-β,and IL-10 in the serum were m easured by ELISA,and the expressions of m icroglial cell and M2 subtype cell m arker in the prim ary m otor cortex were detected using im m unofluorescence staining.ResultsAfter m odeling,com pared with the sham-operation group,the grid error rate and the dragging rate of the affected lim b were increased(P<0.01);the utilization rate of the affected lim b and percentage of the blood perfusion in the affected cortex to healthy side were de⁃creased(P<0.01);the contents of serum IL-1β,TGF-β,and IL-10 were increased(P<0.01,P<0.05);and the m icrog⁃lia in the prim ary m otor cortex on the affected side showed am eboid,the fluorescence intensity of ionized calcium-binding adapter m olecule 1(IBA1)and CD206 was increased(P<0.01)in the m odel group.In the EA group,when com⁃pared with the m odel group,the grid error rate and the dragging rate of affected lim b were decreased(P<0.01);the uti⁃lization rate of affected lim b and the percentage of blood perfusion were increased(P<0.05);the content of serum IL-1βwas decreased(P<0.01),while the contents of TGF-βand IL-10 were increased(P<0.01);and the m icroglia in the pri⁃m ary m otor cortex on the affected side got m ore round and were distributed m ore densely,the fluorescence intensity of IBA1 and CD206 was increased(P<0.01).ConclusionElectroacupuncture preconditioning at“GV20”and“GV14”can up-regulate the expression of m icroglial cells,especially the M2 subtype cell m arker,and increase the contents of the anti-inflam m atory factors and decrease that of the pro-inflam m atory factors in the serum,thereby alleviate the in⁃flam m atory reaction.
作者
焦冰清
骆建宇
姚露露
林舒敏
霍清伟
许沁
许能贵
汤小荣
JIAO Bing-qing;LUO Jian-yu;YAO Lu-lu;LIN Shu-min;HUO Qing-wei;XU Qin;XU Neng-gui;TANG Xiao-rong(School of Public Health and Management,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;South China Research Center for Acupuncture and Moxibustion,Medical College of Acupuncture-moxibustion and Rehabilitation,Guangzhou University of Chinese Medicine,Guangzhou 510006)
出处
《针刺研究》
CSCD
北大核心
2023年第12期1227-1235,共9页
Acupuncture Research
基金
国家自然科学基金青年项目(No.82004469、81904297、81903836)
中医药创新团队及人才支持计划项目(No.ZYYCXTD-C-202004)
广东省重点领域研发计划重点专项(No.2020B1111100008)
中国博士后科学基金(No.2023M740861)。
关键词
缺血性中风
电针预处理
小胶质细胞
炎性反应
Ischem ic stroke
Electroacupuncture preconditioning
M icroglial cells
Inflam m atory response
作者简介
第一作者:焦冰清,博士后,研究方向:针刺效应机制研究。E-mail:jbqscnu@163.com;通信作者:汤小荣,博士后,研究方向:针灸效应机制研究。E-mail:tangxrkk@163.com。
