摘要
该研究以滩羊尾为原料,低温结晶联合尿素包合为方法,共轭亚油酸(conjugated linoleic acid,CLA)得率和纯度为评价指标,在单因素基础上结合正交优化得出最佳提取工艺条件,并探究了滩羊尾脂CLA的生物活性。结果表明,采用低温结晶法提取滩羊尾脂CLA得到的最优条件为:提取溶剂为无水乙醇,结晶温度-20℃,结晶时间12 h,混合脂肪酸与无水乙醇料液比1∶12(g∶mL),提取纯度为(18.62±0.51)mg/g。在此基础上采用尿素包合法,在包合温度25℃,包合时间14 h,脂肪酸与尿素质量比1∶2,尿素与乙醇料液比1∶5(g∶mL)条件下效果最优,其纯度为(28.23±2.36)mg/g。体外抗氧化指标结果显示,DPPH自由基和ABTS阳离子自由基的清除率随滩羊尾脂CLA质量浓度的增加而增加,在质量浓度为6 mg/mL时,清除率分别达到(35.94±4.07)%和(51.76±5.57)%。降胆固醇活性指标结果显示,胆固醇胶束抑制率为(60.33±5.88)%,对牛磺胆酸钠、甘氨胆酸钠和胆酸钠的结合率分别为(16.11±4.01)%、(37.56±3.17)%和(25.11±4.82)%。该结果可为滩羊尾脂CLA工业化提取及活性应用方面的研究提供参考依据。
In this paper,Tan sheep tail was used as raw material,low-temperature crystallization combined with urea inclusion as the method,and the yield and purity of conjugated linoleic acid(CLA)were used as evaluation indicators.The extraction process conditions were investigated,and the biological activity of Tan sheep tail fat CLA was explored.Results showed that the optimal conditions for extracting CLA from Tan sheep tail fat by low-temperature crystallization method were that the extraction solvent was absolute ethanol,the crystallization temperature was-20℃,the crystallization time was 12 h,and the ratio of mixed fatty acids to anhydrous ethanol was 1∶12(g∶mL),the purity was(18.62±0.51)mg/g.On this basis,the urea inclusion method was adopted,when the inclusion temperature was 25℃,the inclusion time was 14 h,the mass ratio of fatty acid to urea was 1∶2,and the mass ratio of urea to ethanol was 1∶5(g∶mL),the effect was best,and the purity was(28.23±2.36)mg/g.The results of in vitro antioxidant indicators showed that the scavenging rates of DPPH radical and ABTS cationic radicals increased with the increase of CLA concentration in Tan sheep tail fat.When the mass concentration was 6 mg/mL,the scavenging rates reached(35.94±4.07)%and(51.76±5.57)%.The results of cholesterol-lowering activity indicators showed that the inhibition rate of cholesterol micelles was(60.33±5.88)%,and the binding rates to sodium taurocholate,sodium glycocholate,and sodium cholate were(16.11±4.01)%,(37.56±3.17)%,and(25.11±4.82)%,respectively.The results provide a reference for future research on the industrial extraction and active application of Tan sheep tail fat CLA.
作者
裴慧敏
李亚蕾
曹松敏
PEI Huimin;LI Yalei;CAO Songmin(School of Food&Wine,Ningxia University,Yinchuan 750021,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2023年第24期132-139,共8页
Food and Fermentation Industries
基金
宁夏自然科学基金项目(2022AAC03078)
河北省重点研发计划项目(21327121D)。
关键词
滩羊尾脂
共轭亚油酸
低温结晶
尿素包合
抗氧化活性
降胆固醇活性
Tan sheep tail fat
conjugated linoleic acid
cryogenic crystallization
urea inclusion
antioxidant activity
cholesterol-lowering activity
作者简介
第一作者:裴慧敏,硕士研究生;通信作者:曹松敏,讲师,E-mail:songmin_cao@126.com。
