摘要
为探究菲(Phenanthrene,Phe)对鱼类的生物毒性效应,本研究以泥鳅(Misgurnus anguillicaudatus)为受试动物,采用亚急性毒性方法将其置于0.15、1.33、1.77、2.36、3.13 mg/L 5个Phe浓度梯度分别暴露15、30、45 d,检测肝脏超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)的活性以及丙二醛(malondialdehyde,MDA)、8-羟基脱氧鸟苷(8-hydroxy-2deoxyguanosine,8-OHdG)的含量,并使用实时荧光定量PCR测定基因Cu/Zn-SOD、CAT3的表达量,之后在自然水体中恢复驯养30 d后再次检测;采用hematoxylin-eosin(H.E)染色法显微观察胁迫30 d泥鳅肝细胞变化情况。结果显示:泥鳅肝脏MDA含量在Phe胁迫初期显著上升,恢复驯养后略有升高但仍低于胁迫组;在同一胁迫时间,8-OHdG含量随浓度升高呈先上升后下降再上升的趋势,恢复期低于胁迫组;SOD活性与Cu/Zn-SOD表达量在胁迫期随浓度增加持续上升,恢复期含量低于胁迫组;CAT活性与CAT3表达量结果相似,在不同时期均显著上升,恢复期含量低于胁迫组;低浓度胁迫30 d,泥鳅肝细胞出现细胞轻微肿大,细胞核溶解、变形、细胞空泡化,随浓度增大细胞发生不同程度坏死。结果表明,Phe胁迫下会对泥鳅肝脏造成一定损伤,四种酶活性、Cu/ZnSOD和CAT3表达量均受毒性显著影响,恢复实验后虽有一定可逆性但并未完全恢复正常。
In order to explore the biotoxic effects of Phenanthrene(Phe)on fish,Misgurnus anguillicaudatus was selected as the subject animal in this study.Subacute toxicity methods were used to expose them to five Phe concentration gradients of 0.15,1.33,1.77,2.36 and 3.13 mg/L for 15,30 and 45 days,respectively.The activity of superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA),8−hydroxy−2 deoxyguanosine(8−OHdG)in liver were detected and the expression levels of Cu/Zn−sod and CAT3 were measured by real-time fluorescence quantitative pcr,and then tested again after 30 days of domestication in natural water.Hematoxylineosin(H.E)staining was used to observe the changes of liver cells of Loaches subjected to stress for 30 days.The results showed that the content of MDA in the liver of Loach increased significantly in the early stage of Phe stress,and slightly increased after domestication,but still lower than that in the stress group.At the same stress time,the content of 8−OHdG increased first,then decreased before increased again with the increase of concentration,and the recovery stage was lower than that of the stress group.Sod activity and Cu/Zn-SOD expression increased continuously with the increase of concentration in the stress stage,and the content in the recovery stage was lower than that in the stress group.The results of CAT activity and CAT3 expression were similar,and both increased significantly in different periods.The content in recovery stage was lower than that in stress group.After 30 days of low concentration stress,the liver cells of Loach showed slight enlargement,nucleolysis,deformation,and cellular vacuolation,and cell necrosis occurred to different degrees with the increase of concentration.The results showed that Phe could cause some damage to the liver of Loach,and the activity of four enzymes,the expression of Cu/Zn-SOD and CAT3 were significantly affected by toxicity.Although there was some reversibility after the recovery experiment,it did not completely return to normal.
作者
王琪
杨超超
明艳
冯宇宇
唐绪
雷忻
WANG Qi;YANG Chaochao;MING Yan;FENG Yuyu;TANG Xu;LEI Xin(School of life sciences,Yan’an University,Yan’an Key Laboratory of Ecological Restoration,Yan’an 716000,China)
出处
《延安大学学报（自然科学版）》
2023年第4期61-68,共8页
Journal of Yan'an University：Natural Science Edition
基金
陕西省自然科学基础研究计划面上项目(2021JM-417)
延安市科技惠民计划项目(2017HM-05)。
关键词
菲
氧化毒性
基因表达
泥鳅
可逆性
Phenanthrene
oxidative toxicity
gene expression
Loach
reversibility
作者简介
王琪(2000-),女,陕西宝鸡人,延安大学硕士研究生;通信作者:雷忻。
