摘要
根据不同品种选择适宜培养基是繁育优良菌种的基础,以平菇品种小白平为试验对象,通过观测菌落形态、菌丝长势和生长速度,比较综合马铃薯葡萄糖琼脂培养基(CPDA)、马铃薯葡萄糖琼脂培养基(PDA)、改良沙氏琼脂培养基(GSDA)、改良蔗糖硝酸钠培养基(GCZA)、苹果培养基(Ap A)、豆芽培养基(BSA)6种供试培养基对小白平母种菌丝生长的影响。结果以BSA培养基的菌丝长势、长速优于其他配方。以BSA为基础培养基,分别以3种碳源(红薯、玉米芯、香蕉皮)替换葡萄糖(30 g/L),3种氮源(茶渣、鹿茸菇菌渣、麸皮)替换蛋白胨(5 g/L)进行菌丝培养试验,以优化培养基碳、氮源,结果显示碳源以葡萄糖、氮源以麸皮表现为佳,从而得到小白平最佳母种培养基配方为新鲜黄豆芽250 g,麸皮5 g,葡萄糖30 g,酵母膏1 g,琼脂20 g,蒸馏水1 000 mL。
Choosing suitable culture media according to different varieties is the foundation for spawn produce,taking Pleurotus ostreatus strain Xiaobaiping as the experimental materials,By observing the colony morphology,mycelial growth potential,and growth rate,the effects of six test media on the mycelial growth of'Xiaobaiping'stock culture were compared,including comprehensive potato glucose agar medium(CPDA),potato glucose agar medium(PDA),improved Sabouraud agar medium(GSDA),improved sucrose sodium nitrate medium(GCZA),apple medium(ApA),and bean sprout medium(BSA).The results showed that the mycelium growth rate and vigor of BSA medium were superior to other formulations.Using BSA as the basic culture medium,glucose(30 g/L)was replaced with three carbon sources(sweet potato,corn cob,and banana peel),and peptone(5 g/L)was replaced with three nitrogen sources(tea residue,pilose antler mushroom residue,and bran)for mycelial culture experiments to optimize the carbon and nitrogen sources of the culture medium.The results showed that glucose was the best carbon source,and bran was the best nitrogen source.Therefore,the optimal stock culture medium formula for strain Xiaobaiping was 250 g fresh soybean sprout and 5 g bran,30 g of glucose,1 g of yeast extract,20 g of agar,and 1000 mL of distilled water.
作者
周光燕
温子欣
张贵合
贺栾劲芝
周星辰
王名炜
程婉莹
赵丹
ZHOU Guangyan;WEN Zixin;ZHANG Guihe;HE Luanjingzhi;ZHOU Xingchen;WANG Mingwei;CHENG Wanying;ZHAO Dan(Guizhou Vocational College of Agriculturre,Guiyang 551400,China;College of Biological Sciences,Guizhou Education University,Guiyang 550018,China)
出处
《食药用菌》
2023年第6期396-400,共5页
Edible and Medicinal Mushrooms
基金
贵州省特色野生食用菌抚育研究黔科合支撑[2020]1Y070号
贵州省食用菌产学研融合项目[2021]02号
贵州省食用菌产学研融合项目[2021]14号
贵州农业职业学院马林大师工作室
贵州农业职业学院院级重点课题(yk23010102)
贵州农业职业学院院级一般课题(yk230111)共同资助。
关键词
平菇
小白平
培养基
母种
碳源
氮源
Pleurotus ostreatus
strain Xiao baiping
culture medium
stock culture
carbon sources
nitrogen sources
作者简介
第一作者:周光燕(1993-),女,硕士,主要从事食用菌栽培研究。E-mail:2253898224@qq.com。;通信作者:张贵合(1988-),男,硕士,省级科技特派员,主要从事食用菌栽培、育种研究。E-mail:610190768@qq.com。
