摘要
目的:通过比较配施不同微生物菌剂后枸杞子中多类型化学成分含量差异,揭示微生物菌剂对枸杞子品质的影响。方法:以宁杞7号为实验材料,通过配施培元和新特锐(TP组)、哈茨木霉和枯草芽孢杆菌(BW组)、根无忧(MT组)和菌益多(JYD组)4种微生物菌剂,以不施微生物菌剂为空白组(CK组),采用紫外分光光度法、高效液相色谱法(HPLC)、超高效液相色谱质谱联用法(UHPLC-MS/MS)测定枸杞子中总酚、总黄酮、糖类、氨基酸、核苷和碱基、甜菜碱等成分含量,运用多重比较、主成分分析(PCA)、偏最小二乘法判别分析(PLS-DA)等方法分析微生物菌剂对枸杞子品质的影响。结果:微生物菌剂对枸杞子中不同的化学成分影响不同,总酚含量以TP组最高,与CK组差异具有统计学意义(P<0.05);总黄酮含量以BW组最高,TP组次之;多糖和糖醛酸含量均以JYD组最高;与CK组比较,TP组和BW组甜菜碱含量均明显升高(P<0.05);测定的23种氨基酸中,大部分氨基酸在JYD组最低,MT组最高;核苷碱基则在MT组和BW组含量较高。基于测定的45种化学成分可以明显区分不同处理组枸杞子。PLS-DA显示,各处理组主要的差异成分为多糖、葡萄糖、果糖、甜菜碱、糖醛酸、天冬酰胺、蔗糖、苏氨酸、总黄酮、丙氨酸和总酚等。基于主要差异成分的PCA综合评分结果显示,各处理组枸杞子化学成分综合得分为BW组>TP组>MT组>CK组>JYD组。结论:配施微生物菌剂BW、TP和MT可以促进枸杞子品质提升,配施JYD在一定程度上促进了多糖和糖醛酸的积累,但对枸杞子品质的整体影响尚不明确。本研究可为枸杞子产业绿色健康发展奠定基础。
Objective:To reveal the effects of different microbial agents on quality of Lycii Fructus by comparing the differences in the contents of multiple types of chemical components in Lycii Fructus after the application of different microbial agents.Method:Taking Ningqi No.7 as experimental material,four microbial agents,namely Peiyuan combined with Xinterui(TP group),Trichoderma harzianum combined with Bacillus subtilis(BW group),Genwuyou(MT group)and Junyiduo(JYD group),were applied,and no microbial agents was used as the blank group(CK group).Then the contents of total phenolics,total flavonoids,saccharides,amino acids,nucleosides and bases,betaine and other components in Lycii Fructus were determined by ultraviolet spectrophotometry(UV),high performance liquid chromatography(HPLC)and ultrahigh performance liquid chromatography-mass spectrometry(UHPLC-MS/MS),and the methods such as multiple comparisons,principal component analysis(PCA)and partial least squares-discriminant analysis(PLS-DA)were used to analyze the effect of different microbial agents on the quality of Lycii Fructus.Result:Microbial agents had different effects on chemical components of Lycii Fructus.The content of total phenolics was the highest in the TP group,and it varied significantly from the CK group(P<0.05).The total flavonoid content was the highest in the BW group,followed by the TP group.Both polysaccharide and alduronic acid contents were the highest in the JYD group.Betaine content in the TP and BW groups were significantly higher than that in the CK group(P<0.05).For the determined 23 kinds of amino acids,most of them were the lowest in the JYD group,and the highest in the MT group,while the nucleoside bases were higher in the MT and BW groups.It indicated that Lycii Fructus from different treatment groups could be distinguished clearly based on the determined 45 chemical components.The result of PLS-DA showed that the major differential components in each group were polysaccharides,glucose,fructose,betaine,alduronic acid,asparagine,sucrose,threonine,total flavonoids,alanine and total phenolics.The results of PCA composite scores based on the main differential components showed that composite scores of chemical components in each group were BW group>TP group>MT group>CK group>JYD group.Conclusion:The application of microbial agents of BW,TP and MT can promote the quality improvement of Lycii Fructus,and the application of JYD can promote the accumulation of polysaccharides and alduronic acid to a certain extent,but the overall effect on the quality of Lycii Fructus is not clear.This study lays the foundation for the green and healthy development of Lycii Fructus industry.
作者
何嘉
卢有媛
谢明霞
孙海霞
王涛
薛佳慧
郝万亮
王汉卿
HE Jia;LU Youyuan;XIE Mingxia;SUN Haixia;WANG Tao;XUE Jiahui;HAO Wanliang;WANG Hanqing(Ningxia Key Laboratory of Plant Disease and Insect Pest Control,Institute of Plant Protection,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,China;Ningxia Regional Characteristic Traditional Chinese Medicine(TCM)Collaborative Innovation Center Co-constructed by the Province and Ministry,Ningxia Engineering and Technology Research Center for Modernization of Regional Characteristic TCM,College of Pharmacy,Ningxia Medical University,Yinchuan 750004,China;College of TCM,Hunan University of Chinese Medicine,Changsha 410007,China;Bairuiyuan Goji Co.Ltd.,Yinchuan 750200,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2023年第24期177-184,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
宁夏农业科技创新引导项目(NKYG-20-03)
宁夏回族自治区重点研发项目(2021BEF02010)。
关键词
微生物菌剂
枸杞子
宁夏枸杞
品质评价
糖类
氨基酸类
核苷和碱基
microbial agents
Lycii Fructus
Lycium bararum
quality evaluation
saccharides
amino acids
nucleoside and nucleobase
作者简介
第一作者:何嘉,硕士,副研究员,从事枸杞植保栽培技术研究,Tel:0951-6882370,E-mail:hejiayc@126.com;通信作者:王汉卿,博士,教授,从事中药资源开发利用研究,Tel:0951-6980193,E-mail:wwwhhq@163.com。
