摘要
土种是土壤发生分类中的最基础分类单元,基于土种的微生物量调查是土壤生物调查中的重要工作,其中微生物量以微生物碳含量表征。采用氯仿熏蒸提取法-碳光谱分析法和重铬酸钾氧化法对比测定土壤中的微生物碳含量,对于提取液中的有机碳含量,碳光谱分析法的检出限为0.11 mg/L,重铬酸钾氧化法的检出限为1.55 mg/L。在精密度实验中,对于熏蒸后的土样,碳光谱分析法的RSD为8.8%~9.9%,重铬酸钾氧化法的RSD为7.0%~17.6%。对于未熏蒸处理的土样,两种方法的RSD分别为5.3%~9.3%和9.3%~12.0%。微生物碳测试结果与土壤的水分含量相关性显著(p<0.05)。对比两种方法的测定结果,发现熏蒸土样的相对偏差为0.1%~49.4%,未熏蒸土样的相对偏差为0~60.9%,低浓度样品的偏差较大。对于不同土种中微生物碳含量的测定,碳光谱分析法测得结果为146~414μg/g dry soil,重铬酸钾氧化法为131~407μg/g dry soil,两种方法测定结果间无显著差异。结果表明,低浓度样品测定时采用重铬酸钾氧化法造成的误差较大,宜采用碳光谱分析法,两种方法均能有效测定不同土种中的微生物碳含量。
Soil species is the most basic unit in soil classification,and soil microbial biomass survey based on soil species is important in soil biological survey,in which microbial biomass is characterized by microbial biomass carbon content.The chloroform fumigation extraction method-carbon spectroscopy analysis method and potassium dichromate oxidation method were used to compare and determine the microbial carbon content in soil.For the organic carbon content in the extraction solution,the detection limit of carbon spectroscopy analysis method was 0.11 mg/L,and the detection limit of potassium dichromate oxidation method was 1.55 mg/L.In the precision test,for the soil sample after fumigation,the RSD of carbon spectroscopy analysis method was 8.8%—9.9%,and the RSD of potassium dichromate oxidation method was 7.0%—17.6%.While for soil samples without fumigation treatment,the RSD of the two methods were 5.3%—9.3%and 9.3%—12.0%,respectively.The correlation between microbial carbon test results and soil moisture content was significant(p<0.05).Comparing the measurement results of the two methods,it was found that the relative deviation range of fumigated soil samples was 0.1%—49.4%,while the relative deviation range of non fumigated soil samples was 0—60.9%.There was large deviation of low concentration samples.For the determination of microbial carbon content in different soil species,the range of results obtained by carbon spectroscopy analysis was 146—414μg/g dry soil,and the range of potassium dichromate oxidation method was 131—407μg/g dry soil.There was no significant difference in the results between the two methods.The results indicated that the use of potassium dichromate oxidation method in the determination of low concentration samples resulted in significant errors,and carbon spectroscopy analysis should be used.Both methods can effectively determine the microbial carbon content in different soil species.
作者
范雪滢
赖伟
徐志锐
梁诗捷
邓豪健
梅承芳
FAN Xueying;LAI Wei;XU Zhirui;LIANG Shijie;DENG Haojian;MEI Chengfang(Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou,Guangdong 510070,China;Guangdong Detection Center of Microbiology,Guangzhou,Guangdong 510070,China;State Key Laboratory of Applied Microbiology Southern China,Guangzhou,Guangdong 510070,China;Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangzhou,Guangdong 510070,China)
出处
《中国无机分析化学》
CAS
北大核心
2023年第12期1389-1395,共7页
Chinese Journal of Inorganic Analytical Chemistry
基金
广东省科学院打造综合产业技术创新中心行动资金资助项目(2022GDASZH-2022010105)
广东省第三次土壤普查土壤生物调查项目(GPCGD232200FG091F)。
关键词
熏蒸提取法
微生物碳
土种
碳光谱分析法
重铬酸钾氧化法
fumigation extraction method
microbial biomass carbon
soil species
carbon spectroscopy analysis
potassium dichromate oxidation method
作者简介
范雪滢,女,工程师,主要从事环境监测、生态毒理和农业生态研究。E-mail:fanxy@gddcm.com。
