摘要
为探究烟草青枯病发生与土壤生态环境因子间的关系,明确与青枯病发病程度相关的因素,通过田间调查收集青枯病不同发病程度的根际土壤,测定土壤理化指标和酶活性并用16S DNA、内转录间隔区(Internally transcribed spacer, ITS)基因测序技术分析烟株发病与未发病植株根际土壤细菌、真菌群落结构的差异。结果表明,在烟株发生青枯病的根际土壤中,随着烟株发病程度的加重,土壤pH值、有机质含量、总氮含量降低,硝态氮(NO_(3)^(-)-N)含量升高;发病烟株根际土壤真菌群落中镰刀菌属(Fusarium)、毛霉属(Mucor)、Pseudaleuria与细菌群落中肠杆菌属(Enterobacter)、鞘氨醇单胞菌属(Sphingomonas)的相对丰度高于正常烟株根际土壤;通过LEFse及相关性分析发现,柱孢霉菌属(Cylindrocarpon)、毛霉属(Mucor)、鞘氨醇单胞菌属(Sphingomonas)可能是不同青枯病发病程度烟株根际土壤中微生物在属水平产生差异的主要物种;冗余分析(RDA)结果表明,总氮含量、有机质含量可能是影响烟草青枯病发生的关键土壤因子。综上所述,土壤总氮含量、有机质含量与鞘氨醇单胞菌属(Sphingomonas)、毛霉属(Mucor)微生物相对丰度的降低以及柱孢霉菌属微生物相对丰度的增加是引起烟草青枯病严重发生的关键因素。
In order to explore the relationship between the occurrence of tobacco wilt and soil ecological and environmental factors,and to clarify the factors related to the incidence of wilt,rhizosphere soil with different degrees of wilt was collected through field investigation,soil physicochemical indices and enzyme activities were measured,and the differences of bacterial and fungal community structure in rhizosphere soil of pathogenic and non-diseased tobacco plants were analyzed by 16S DNA and internally transcribed spacer(ITS)gene sequencing technology.The results showed that in the rhizosphere soil of tobacco plants with bacterial wilt,with the aggravation of the degree of disease,the soil pH value,organic matter content and total nitrogen content decreased,and the content of nitrate nitrogen(NO_(3)^(-)-N)increased.The relative abundance of Fusarium,Mucor and Pseudaleuria in the fungal community and Enterobacter and Sphingomonas in the bacterial community in the rhizosphere soil of diseased tobacco plants was higher than that in the rhizosphere soil of normal tobacco plants.Through LEFse and correlation analysis,it was found that Cylindrocarpon,Mucor,and Sphingomonas may be the main different species of microorganisms in the rhizosphere soil of tobacco plants with different degrees of wilt at the genus level.The results of redundancy analysis(RDA)showed that total nitrogen content and organic matter content may be the key soil factors affecting the occurrence of wilt.In summary,the decrease of soil total nitrogen content,organic matter content and relative abundance of Sphingomonas and Mucor,and the increase of relative abundance of Cylindrocarpon were the key factors causing the serious occurrence of wilt.
作者
白茂军
高正锋
张力元
范成平
潘首慧
董延鑫
杨索
王莹
陈汶
杨小龙
岑浩
田玉琴
昝建朋
吴海
BAI Mao-jun;GAO Zheng-feng;ZHANG Li-yuan;FAN Cheng-ping;PAN Shou-hui;DONG Yan-xin;YANG Suo;WANG Ying;CHEN Wen;YANG Xiao-long;CEN Hao;TIAN Yu-qin;ZAN Jian-peng;WU Hai(Anshun Branch of Guizhou Tobacco Company,Anshun 561000,China;Yunnan Agricultural University,Kunming 650201,China;China Tobacco Guizhou Industrial Co.,Ltd.,Guiyang 550009,China)
出处
《江苏农业学报》
CSCD
北大核心
2023年第6期1294-1302,共9页
Jiangsu Journal of Agricultural Sciences
基金
贵州省烟草公司重点研发项目(2021XM15)
云南省教育厅科学研究基金项目(2022Y250)。
关键词
烟草青枯病
土壤理化性状
酶活性
细菌
真菌
tobacco bacterial wilt
soil physical and chemical properties
enzyme activity
bacteria
fungi
作者简介
白茂军(1983-),男,重庆人,助理农艺师,农业推广硕士,从事烤烟种植与收购工作。(E-mail)772390297@qq.com;通讯作者:张力元,(E-mail)760061799@qq.com。
