摘要
以木薯(Manihot esculenta)‘TMS60444’脆性胚性愈伤组织(FEC)为材料,研究了在GD基础培养基中添加不同浓度外源褪黑素(melatonin)对FEC增殖的影响。结果表明:在不含氨氯吡啶酸(4-氨基-3,5,6-三氯吡啶-2-羧酸)的GD培养基(GD0)中加入不同浓度褪黑素,其中低浓度(<100μmol·L^(-1))褪黑素可以促进FEC增殖,但高浓度褪黑素使FEC生长缓慢并褐化。在含有12 mg·L^(-1)氨氯吡啶酸的GD培养基(GD1)中添加不同浓度褪黑素(0、0.1、1、10、100μmol·L^(-1))进行培养,FEC鲜重增长率明显不同;以添加10μmol·L^(-1)褪黑素效果最佳,可使FEC保持较好的增殖状态,并显著提高木薯FEC鲜重的增长率,比不加褪黑素的GD1-1培养基中增加了2.57倍。用10μmol·L^(-1)褪黑素处理木薯FEC,其内源褪黑素代谢通路相关基因表达发生改变,抗氧化相关基因明显上调表达;并且抗氧化酶活性显著增强,抗氧化能力明显提高。本研究为木薯遗传转化中延长木薯FEC使用周期和提高增殖效率提供思路和技术。
To investigate the effect of melatonin on friable embryogenic callus(FEC)proliferation in cassava(Manihot esculenta)‘TMS60444',GD media with different melatonin levels were tested for FEC subculturing.When GD media was without picloram(4-amino-3,5,6-trichloropyridine-2-carboxylic acid)(GD0),supplementing with low concentrations of melatonin(less than 100μmol·L^(-1))showed a positive effect on FEC proliferation.Meanwhile,FEC showed inhibited growth and browning in the GD0 medium with higher levels of melatonin.When GD media was supplemented with 12 mg·L^(-1)picloram(GD1)and different levels of melatonin(0,0.1,1,10,and 100μmol·L^(-1)),the proliferation rates of FEC were variable and 10μmol·L^(-1)melatonin was the most suitable concentration for better growth,showing a 2.57-fold increase of fresh mass that of GD1-1 control.Under the 10μmol·L^(-1)melatonin treatment,there was altered expression in melatonin biosynthetic genes and upregulated expression of antioxidant enzyme genes in FEC,leading to improved antioxidant capacity by increased ROS-scavenging enzymatic activity.Our study provides a practical approach for prolonging the viability of cassava FEC during genetic transformation.
作者
程露
郑偲葶
陆新露
张鹏
马秋香
CHENG Lu;ZHENG Siting;LU Xinlu;ZHANG Peng;MA Qiuxiang(National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2023年第10期1881-1889,共9页
Plant Physiology Journal
基金
国家重点研发计划(2019YFD1001100)
国家自然科学基金(31801417)
农业农村部国家现代农业产业技术体系(CARS-11)项目。
关键词
木薯
褪黑素
脆性胚性愈伤
增殖
抗氧化
cassava
melatonin
friable embryogenic callus
proliferation
antioxidation
作者简介
并列第一作者:程露;并列第一作者:郑偲葶;通信作者:马秋香,qxma@cemps.ac.cn。
