摘要
目的从追溯辐射引起的DNA损伤机制出发,根据基本的生物物理原理获取DNA双链断裂(DSB)损伤簇表示的相对生物效能(RBE)值,探讨DNA损伤与染色体畸变、生殖细胞死亡生物效能的相似性。方法以低能电子、质子、α粒子作为研究对象,通过辐射物理化学模型,模拟细胞核受粒子辐射的过程。在基于损伤点密度的含噪声密度聚类(DBSCAN)算法的基础上,改进DSB损伤簇分类方法,以减弱模拟过程中损伤点与能量沉积随机分布假设的联系,使得DSB损伤簇更接近于非随机分布。计算获得DSB损伤簇的产额,提出以DSB损伤簇计算RBE值的方法。结果2 MeVα粒子的DSB损伤簇RBE值(12.29)与生物实验中染色体片段(15.3±5.9)、细胞存活(14.7±5.1)表示的RBE相近。结论高传能线密度(LET)电离辐射后,不同于简单DSB,DSB损伤簇的RBE与染色体畸变、细胞存活生物效能存在一定的相似性。
Objective To obtain the relative biological effectiveness(RBE)of DNA double strand breaks(DSB)clusters by tracing the mechanism of radiated DNA damage,and explore the relationship among the biological effectiveness of DNA damage,chromosomal aberrations and germ cell death.Methods Taking low-energy electrons,protons,andαparticles as the research objects,this study simulated the process that cell nuclei were exposed to particle radiation using a radiation-related physicochemical model.On the ground of the DSB density-based spatial clustering of applications with noise(DBSCAN)algorithm,the DSB cluster classification method was improved to weaken the connection between the DSBs and the random distribution assumptions of energy depositions during the simulation.In this manner,the DSB clusters can be much closer to a non-random distribution.Furthermore,this study obtained the yields of DSB clusters and proposed a method to calculate the RBE values of DSB clusters.Results The calculated RBE value(12.29)of DSB clusters of 2 MeVαparticles was similar to the experimental RBE values of chromosomal fragments(15.3±5.9)and cell survival(14.7±5.1).Conclusions After high-LET ionizing radiation,unlike the single DSB,the RBE of DSB clusters was similar to that of chromosomal aberration and cell survival.
作者
唐菁
马骥祥
李志媛
李杰
张鹏程
Tang Jing;Ma Jixiang;Li Zhiyuan;Li Jie;Zhang Pengcheng(Shanxi Provincial Key Laboratory for Biomedical Imaging and Big Data,North University of China,Taiyuan 030051,China;School of Software,North University of China,Taiyuan 030051,China;School of Information and Communication Engineering,North University of China,Taiyuan 030051,China;Department of Radiation Oncology,Shanxi Provincial Cancer Hospital,Taiyuan 030013,China)
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2023年第7期489-498,共10页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金(11605160)。
关键词
DNA双链断裂损伤簇
相对生物效能
含噪声密度聚类算法
DNA double strand break clusters
Relative biological effectiveness
Densitybased spatial clustering of applications with noise algorithm
作者简介
通信作者:张鹏程,Email:zhangpc198456@163.com。
