摘要
氨基酸是水中重要的含氮有机物,已有研究表明,水中含氮消毒副产物的前体,其中一些具有细胞毒性和致癌性。因此,相关人员开发了一种固相萃取-超高效液相色谱-串联质谱法(UPLC-MS/MS)测定15种游离氨基酸(FAAs),并成功应用于饮用水源水样品的测试。研究结果表明:强阳离子交换固定固相萃取柱的萃取性能优于反相固相萃取柱,提取前确定水的最佳pH值为2.8,所以建议用SPE提取样品以消除基质效应。所开发的方法显示出良好的线性(R^(2)>0.991),检测限低(LODs,0.01~0.27 nmol/L),在饮用水源水中的回收率为69.8%~117.9%。
Amino acids are important nitrogen-containing organic compounds in water.Previous studies have shown that they are precursors of nitrogen-containing disinfection by-products in water,some of which have cytotoxicity and carcinogenicity.Therefore,a solid-phase extraction ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method for the determination of 15 free amino acids(FAAs)was developed and successfully applied to the testing of drinking water samples.The research results indicate that the extraction performance of the strong cation exchange fi xed solid-phase extraction column is superior to that of the reverse phase solid-phase extraction column,the optimal water pH determined before extraction is 2.8,therefore,it is recommended to extract samples using SPE to eliminate matrix effects,the developed method exhibits good linearity(R^(2)>0.991),low detection limits(LODs,0.01~0.27 nmol/L),and a recovery rate of 69.8%~117.9%in drinking water sources.
作者
赵妮娜
Zhao Nina(Guangyuan Ecological Environment Monitoring Center Station,Sichuan Province,Guangyuan 628017,China)
出处
《皮革制作与环保科技》
2023年第18期88-90,共3页
Leather Manufacture and Environmental Technology
关键词
固相萃取
液相色谱
串联质谱法
氨基酸
污染物
solid phase extraction
liquid chromatography
tandem mass spectrometry
amino acids
pollutant
作者简介
赵妮娜(1987-),女,本科,工程师,研究方向:环境监测中水、土壤、空气中重金属及有机污染物的检测。
