摘要
从内蒙古自治区通辽市部分规模化养牛场采集粪样350份、血样325份,分别采用RT-PCR和ELISA方法进行牛冠状病毒(Bovine coronavirus,BCoV)的分子流行病学调查,了解内蒙古自治区通辽市规模化养殖场牛冠状病毒的流行状况,以期为通辽地区养牛场牛冠状病毒病的防控提供参考。结果显示:抗原总阳性率为22.86%,抗体总阳性率为7.69%。在采集的样品中,0~2月龄犊牛的抗原阳性率最高,达57.50%,抗体阳性率最高,达19.00%,表明通辽地区牛冠状病毒流行率较高,及时采取科学有效的防控措施是很有必要的。
In order to understand the epidemic situation of Bovine coronavirus disease in large-scale farms in Tongliao City,Inner Mongolia Autonomous Region,350 manure samples and 325 serum samples were collected from some large-scale cattle farms in Tongliao City,Inner Mongolia Autonomous Region.Molecular epidemiological investigation of Bovine coronavirus(BCoV)was performed using RT-PCR and ELISA methods for fecal samples and serum,respectively.The results showed that the total positive rate of antibody was 7.69%,and the total positive rate of antigen was 22.86%.Among the samples collected,calves aged 0~2 months had the highest antigen positivity rate,reaching 57.50%,and antibody positivity rate was the highest,reaching 19.00%,indicating that the prevalence of bovine coronavirus in Tongliao City was high,and it was necessary to take scientific and effective prevention and control measures in time.
作者
石晓娇
谢梦圆
张园园
郭宇
萨茹拉
陈柯佳
尚和卫
郝彦儒
许燕飞
徐晓静
SHI Xiaojiao;XIE Mengyuan;ZHANG Yuanyuan;GUO Yu;SA Rula;CHEN Kejia;SHANG Hewei;HAO Yanru;XU Yanfei;XU Xiaojing(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Animal Agriculture Association,Hohhot O10000,China;Inner Mongolia Animal Disease Control Center,Hohhot 010020,China)
出处
《兽医导刊》
2023年第2期20-23,共4页
Veterinary Orientation
基金
呼和浩特市重大科技专项(2022-农-重-1-1)
呼和浩特市科技创新领域人才项目(2022RC-联合体-1)
内蒙古自治区科技重大专项“牛腹泻疾病防控关键技术研发与标准化操作规程示范推广”(2020ZD0006)
内蒙古自治区科技重大专项“牛羊支原体肺炎和病毒性呼吸道疾病的防控技术研发与示范”(2021ZD0013)。
作者简介
石晓娇(1998-),女,内蒙古自治区通辽市人,硕士研究生,研究方向为动物组织胚胎发育及疫病防控,E-mail:1462124496@qq.com;通信作者:徐晓静,E-mail:xuxiaojing72@sina.com。
