摘要
目的:本课题旨在通过网络药理学手段,对“生肌愈疮膏”外敷治疗压力性溃疡的机制进行探讨。方法:采用现代中医药理论与技术,以中药为研究对象,采用DisGeNET、GeneCards、Human孟德尔基因编辑技术等技术,对其进行深入研究,寻找其可能的药效物质基础与作用靶标。采用Cytoscape 3.9.1软件,建立“药效-成分-靶点-病变”的药效关系,利用STRING数据库,建立“靶蛋白-效应关系”的效应关系,并利用DAVID数据,对“药效关系”的信号途径进行富集分析。结果:经网络拓扑分析,木犀草素(MOL000006)、毒扁豆次碱(MOL013287)、山奈酚(MOL000422)、槲皮素(MOL000098)、黄芩素(MOL002714)、汉黄芩素(MOL000173)是生肌愈疡膏干预压力性溃疡的主要有效成分。生肌愈疡膏外用可能通过调控PTGS1、PTGS2、SCN5A、AR等基因,介导糖尿病并发症AGERAGE信号通路、动脉粥样硬化通路和癌症通路等通路干预压力性溃疡。结论:生肌愈疡膏可通过调控多靶点、多通路发挥抗炎、抗氧化、促进血管新生等方面促进压力性溃疡创面的愈合。
Objective:The aim of this project was to investigate the mechanism of the Shengji Yuyang Ointment topical treatment of pressure ulcers by means of network pharmacology.Method:Using modern Chinese medicine theories and techniques,DisGeNET,GeneCards,and Human Mendelian gene editing techniques were used to investigate the possible pharmacological bases and targets of the Chinese medicine.Cytoscape 3.9.1 software was used to establish the pharmacodynamic relationship of"pharmacodynamic-component-target-lesion",STRING database was used to establish the effect relationship of"target protein-effect relationship",and DAVID data was used to enrich the signal pathways of"pharmacodynamic relationship".Result:By network topology analysis,Lignan(MOL000006),Poisonous Lentil Hypocretin(MOL013287),Kaempferol(MOL000422),Quercetin(MOL000098),and Baicalein(MOL002714),Wogonin(MOL000173)were the main active ingredients of the Shengji Yuyang Ointment to intervene in pressure ulcers.The topical application of Shengji Yuyang Ointment may interfere with pressure ulcers by regulating PTGS1,PTGS2,SCN5A,AR and other genes and mediating AGE-RAGE signaling pathway in diabetic complications,atherosclerotic pathway and cancer pathway.Conclusion:Shengji Yuyang Ointment can promote the healing of pressure ulcer wounds by modulating multiple targets and pathways,such as anti-inflammation,antioxidation and promotion of vascular renewal.
作者
薛天寅
邵玉芳
孙庆
XUE Tianyin;SHAO Yufang;SUN Qing(The First Clinical School of Shandong University of Traditional Chinese Medicine,Jinan 250014,China;不详)
出处
《中国医学创新》
CAS
2023年第26期169-174,共6页
Medical Innovation of China
基金
济南市卫生健康委员会中医药科技计划项目(2021—中—05)。
关键词
网络药理学
生肌愈疡膏
压力性溃疡
信号通路
作用靶点
Network pharmacology
Shengji Yuyang Ointment
Pressure ulcer
Signaling pathway
Target of action
作者简介
通信作者:孙庆。
