摘要
【目的】明确火龙果茎溃疡病对火龙果植株茎组织真菌群落的影响,为揭示火龙果溃疡病发生机制及防治技术研究提供理论依据。【方法】采集火龙果健康植株和溃疡病发病植株茎组织,利用Illumina高通量测序平台对植株茎组织真菌rDNA的ITS序列进行测序,分析健康植株与发病植株茎组织中的真菌群落特征差异。【结果】火龙果健康植株茎组织的真菌群落多样性显著高于发病植株(P<0.05,下同),且健康植株与发病植株茎组织的真菌群落组成具有明显差异。与健康植株相比,发病植株茎组织的子囊菌门(Ascomycota)和担子菌门(Basidiomycota)相对丰度较高,分别为49.11%和10.90%,门分类水平的未知真菌(unclassified_k_Fungi)的相对丰度较低(39.97%)。在属分类水平上,发病植株茎组织存在8个优势属,其中柱节孢属(Neoscytalidium)的相对丰度最高(33.29%),汉纳酵母属(Hannaella)、Dirkmeia、丛赤壳科未知属(unclassified_f_Nectriaceae)和刺盘孢属(Colletotrichum)4个优势属的相对丰度显著高于健康植株,未在健康植株检测到柱节孢属、维希尼克氏酵母属(Vishniacozyma)和Meira;健康植株茎组织存在4个优势属,其中地霉属(Geotrichum)和链格孢属(Alternaria)的相对丰度显著高于发病植株。线性判别分析(LEfSe)结果显示,发病植株的柱节孢属和维希尼克氏酵母属以及健康植株的地霉属最能解释健康植株与发病植株间的差异。通过比对FUNGuild数据库预测真菌的营养型,发现发病植株中丰度最高的营养型为病理营养型(15.46%),健康植株中丰度最高的营养型是腐生营养型(7.95%)。【结论】火龙果发生茎溃疡病后其茎组织的真菌群落多样性显著下降,真菌群落结构发生改变。当病原营养型真菌显著富集在茎组织中时会破坏茎组织的菌群平衡,可能促进了茎溃疡病的发生。
【Objective】This study clarified the influence of canker on the fungal community in stem tissues of pitaya,to provide theoretical reference for the research on the occurrence and the prevention and control of pitaya canker.【Method】The stem tissues of healthy and canker diseased plants of pitaya were collected,and the ITS sequences of rDNA of fungi in stem tissue were sequenced using Illumina high-throughput sequencing platform,so as to analyze the characteristics and differences of fungal communities in the stem tissues of healthy and diseased plants.【Result】The diversity of fungal community in stem tissues of healthy plants was significantly higher than that of diseased plants(P<0.05,the same below),and the composition of fungal community in stem tissues of healthy plants was greatly different from that of di-seased plants.Compared with healthy plants,the relative abundance of Ascomycota and Basidiomycota in stem tissues of diseased plants was higher,which were 49.11%and 10.90%,while that of unclassified_k__Fungi at the phylum level was lower(39.97%).At the genus level,there were 8 dominant genera in the stem tissue of the diseased plants.And the rela-tive abundance of Neoscytalidium was the highest(33.29%).The relative abundance of 4 dominant genera,including Hannaella,Dirkmeia,unclassified_f__Nectriaceae,and Colletotrichum was significantly higher than that of the healthy plants,while Neoscytalidium,Vishniacozyma and Meira were not detected in healthy plants.There were 4 dominant genera in the stem tissues of healthy plants,of which the relative abundance of Geotrichum and Alternaria was significant-ly higher than that of diseased plants.The results of linear discriminant analysis effect size(LEfSe)showed that the diffe-rence between healthy and diseased plants could be best explained by Neoscytalidium and Vishniacozyma in diseased plants and Geotrichum in healthy plants.By analyzing the FUNGuild database to predict the trophic types of fungi,it was found that pathotroph(15.46%)contained the highest abundance in diseased plants,while saprotroph(7.95%)contained the highest abundance in healthy plants.【Conclusion】When the pitaya canker occurred,the diversity of fungal communi-ty decreased significantly,and the composition of fungal community changed.When fungi of the pathotroph enriched sig-nificantly in stem tissues,the balance of the fungal community would be disturbed and probably trigger stem canker.
作者
姚姿婷
贤小勇
李瑞芳
韦小妹
邹承武
朱桂宁
YAO Zi-ting;XIAN Xiao-yong;LI Rui-fang;WEI Xiao-mei;ZOU Cheng-wu;ZHU Gui-ning(Plant Protection Research Institute,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs,Nanning,Guangxi 530007,China;College of Agriculture,Guangxi University,Nanning,Guangxi 530004,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2023年第5期1283-1292,共10页
Journal of Southern Agriculture
基金
广西自然科学基金项目(2023GXNSFAA026271)
广西农业科学院基本科研业务专项(桂农科2023YM05,桂农科2021YT063)
广西农业科学院科技发展基金项目(桂农科2021ZX28)。
作者简介
第一作者:姚姿婷(1983-),博士,副研究员,主要从事特色果蔬病害发生与综合防治研究工作。主持国家自然科学基金青年基金项目“真菌病毒FsCV1介导甘蔗镰孢菌致病力衰退的分子机制研究”和广西自然科学基金项目“内生菌对火龙果溃疡病的生防潜力及其作用机制研究”,作为主要成员参与国家自然科学基金项目“14-3-3蛋白调控轮枝镰刀菌的菌丝生长和致病性的分子机制”等8项。在《Frontiers in Microbiology》《Biotechnology for Biofuels》《Plant Disease》《Current Genetics》《植物病理学报》《生物技术通报》《南方农业学报》等国内外期刊发表学术论文30余篇,参与获得国家发明专利6项,荣获广西高等教育自治区级教学成果奖三等奖1项,https://orcid.org/0000-0001-5780-338X,E-mail:youziting@163.com;通讯作者:朱桂宁(1968-),https://orcid.org/0000-0002-6719-9961,博士,研究员,主要从事植物病理学及杀菌剂研究工作,E-mail:zgn@gxaas.net。
