摘要
目的:研究海盐弗朗西斯菌( Francisella salimarina)的系统发育和毒力基因分布情况。 方法:纳入GenBank数据库中的相关基因组数据,对海盐弗朗西斯菌及邻近菌种进行系统发育分析,分析16S rRNA基因、 rpoB基因、 mdh基因等单个基因与核心基因组系统发育的一致性,基于毒力因子数据库和抗生素耐药基因数据库注释并分析毒力基因及耐药基因。以蜃楼弗朗西斯菌( Francisella philomiragia) ATCC 25015 T为参考,采用大蜡螟幼虫感染试验评估海盐弗朗西斯菌的毒力。 结果:海盐弗朗西斯菌在系统发育上与蜃楼弗朗西斯菌亲缘关系最为相近。系统发育树比较发现, mdh基因系统发育树与核心基因组系统发育树高度相似。而单基因系统发育分析显示,基于16S rRNA基因和 mdh基因序列分析均能鉴别海盐弗朗西斯菌及邻近菌种,但 mdh基因具有更强的区分能力。8株海盐弗朗西斯菌共检出多种毒力基因,主要与分泌系统、黏附、免疫调节、运动和应激生存相关。此外,8株菌均检测到β-内酰胺类耐药基因 blaFPH。该菌在大蜡螟幼虫感染试验中表现出较高的致死能力,与蜃楼弗朗西斯菌ATCC 25015 T相近。 结论:海盐弗朗西斯菌是一种致病能力与蜃楼弗朗西斯菌相近的罕见病原体, mdh基因可作为快速鉴定海盐弗朗西斯菌的分子靶标。
Objective To study the molecular phylogeny and virulence gene profile of Francisella salimarina.Methods Phylogenetic analysis of Francisella salimarina was performed based on the global genome data of related Francisella species on GenBank database.The consistency in phylogenetic analysis based on single marker genes(such as 16S rRNA gene,rpoB gene and mdh gene)and the core genome as compared.Virulence genes and antibiotic resistance genes were annotated using the virulence factor database(VFDB)and the Comprehensive Antibiotic Resistance Database(CARD),respectively.The virulence of Francisella salimarina was analyzed with a Galleria mellonella(greater wax moth)infection model using Francisela philomiragia ATCC 25015'as reference strain.Results The phylogenetic analysis revealed that Francisella salimarina was closely related to Francisella philomiragia.The phylogenetic tree based on mdh gene was highly similar to that based on the core genome.Francisella salimarina could be differentiated from other related species by 16S rRNA gene or mdh gene,with the latter being more accurate.Eight Francisella salimarina strains carried multiple virulence genes,mainly involved in secretion,adhesion,immune regulation,motility and stress survival.Moreover,beta-lactam resistance gene blappH was identified in all eight strains.Francisella salimarina showed high lethality in the Galleria mellonella infection model,which was similar to Francisella philomiragia ATCC 25015T.Conclusions Francisella salimarina was a rare pathogen with similar pathogenicity to Francisella philomiragia.The mdh gene could be used as a molecular target for rapid identification of Francisella salimarina.
作者
陈晓慰
李启伟
陈燕
李顺光
陈家帆
杨超
陈茶
屈平华
蔡壬辛
Chen Xiaowei;Li Qiwei;Chen Yan;Li Shunguang;Chen Jiafan;Yang Chao;Chen Cha;Qu Pinghua;Cai Renxin(The Second Clinic Medical College,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Traditional Chinese Medicine Innovation Research Center,Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine,Shenzhen 518104,China;The Second Clinic Medical College,Guangzhou University of Chinese Medicine/Department of Laboratory Medicine,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou 510006,China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2023年第8期612-618,共7页
Chinese Journal of Microbiology and Immunology
基金
国家科技部基础资源调查专项基金(2021FY100900)。
关键词
海盐弗朗西斯菌
系统发育
毒力基因
致病性
Francisella salimarina
Molecular phylogeny
Virulence gene
Pathogenicity
作者简介
通信作者:蔡壬辛,Email:cairengxing@163.com,电话:020-39318321。
