摘要
本试验旨在研究纳米氧化锌(N-ZnO)对仔猪血液生化、抗氧化、免疫指标及游离氨基酸含量的影响。选取21日龄杜×长×大断奶仔猪[(6.15±0.01) kg] 144头,随机分为6组,每组6个重复,每个重复4头仔猪。抗生素(ANTI)组在基础饲粮中添加56 g/t金霉素,氧化锌(ZnO)组在基础饲粮中添加1 600 mg/kg ZnO,N-ZnO组在基础饲粮中分别添加200、300、400、500 mg/kg N-ZnO (分别为N200、N300、N400、N500组)。26 d后,采集仔猪血液,测定血浆生化、抗氧化指标及游离氨基酸含量以及血清免疫球蛋白(Ig)含量。结果表明:1)与ANTI组和ZnO组相比,N-ZnO组血浆谷草转氨酶活性显著降低(P<0.05);ZnO组血浆谷丙转氨酶、碱性磷酸酶活性显著高于ANTI组和N-ZnO组(P<0.05)。2)ANTI组、ZnO组、N500组血浆谷胱甘肽过氧化物酶活性显著高于其余各组(P<0.05);与ZnO组和N500组相比,N200~N400组血浆总抗氧化能力显著降低(P<0.05)。3)与ANTI组相比,N200~N400组和ZnO组血清IgA含量显著提高(P<0.05);与ANTI组和ZnO组相比,N200~N300组的血清IgG含量以及N400组的血清IgM含量显著提高(P<0.05)。随着N-ZnO添加量的增加,血清中IgA、IgG含量呈线性降低和二次曲线变化(P<0.05),血清IgG含量呈线性增加和二次曲线变化(P<0.05)。4)与ANTI组和ZnO组相比,N200~N400组血浆组氨酸含量显著提高(P<0.05),血浆脯氨酸、β-丙氨酸、羟脯氨酸含量显著降低(P<0.05)。随着N-ZnO添加量的增加,仔猪血浆精氨酸和瓜氨酸含量呈二次曲线变化(P<0.05),丝氨酸、甘氨酸、羟脯氨酸含量呈二次曲线变化或线性增加(P <0.05)。综上所述,饲粮添加N-ZnO对仔猪肝脏功能、抗氧化能力、免疫功能产生了一定益处,还改变了血浆游离氨基酸谱。
This study investigated the effects of nano zinc oxide(N⁃ZnO)on blood biochemical,antioxidant and immune indexes and free amino acid contents in piglets.A total of 144 piglets[Duroc×Landrace×Large White,weaned at 21 days of age;initial weight(6.15±0.01)kg]were assigned to 6 groups with 6 replicates in each group and 4 pigs per replicate.The groups were:antibiotic(ANTI)group(basal diet+75 mg/kg aure⁃omycin),zinc oxide(ZnO)group(basal diet+1600 mg/kg ZnO),and N⁃ZnO groups(basal diet+200,300,400 or 500 mg/kg N⁃ZnO,named as N200,N300,N400 and N500 groups,respectively).After 26 d,the blood was collected to determine plasma biochemical and antioxidant indexes and free amino acid contents,as well as serum immunoglobulin(Ig)contents.The results showed as follows.1)compared with ANTI and ZnO groups,the plasma aspartate aminotransferase activity was significantly decreased in N⁃ZnO groups(P<0.05),and the activities of alanine aminotransferase and alkaline phosphatase were significantly higher in ZnO group than those in ANTI and N⁃ZnO groups(P<0.05).2)The plasma glutathione peroxidase activity was significantly higher in ANTI,ZnO and N500 groups than that in the rest groups(P<0.05).Compared with ZnO and N500 groups,plasma total antioxidant capacity was significantly decreased in N200 to N400 groups(P<0.05).3)Compared with ANTI group,the serum IgA content was significantly increased in N200 to N400 groups and ZnO group(P<0.05).Compared with ANTI and ZnO groups,the serum IgG content in N200 to N300 groups and the IgM content in N400 group were significantly increased(P<0.05).With the in⁃crease of N⁃ZnO additive amount,the contents of IgA and IgG in serum were decreased linearly and quadrati⁃cally(P<0.05),and IgG content was increased linearly and quadratically(P<0.05).4)Compared with AN⁃TI and ZnO groups,the plasma histidine content was significantly increased in N200 to N400 groups(P<0.05),and the contents of proline,β⁃alanine,and hydroxyproline were significantly decreased(P<0.05).With the increase of N⁃ZnO additive amount,the contents of arginine and citrulline in plasma were decreased quadratically(P<0.05),while the contents of serine,glycine and hydroxyproline were increased quadratically or linearly(P<0.05).In conclusion,dietary N⁃ZnO improves the liver function,antioxidant capacity,im⁃mune function,and alters free amino acid profile in plasma of piglets.
作者
崔艺燕
田志梅
刘志昌
容庭
李贞明
余苗
马现永
叶秀峰
CUI Yiyan;TIAN Zhimei;LIU Zhichang;RONG Ting;LI Zhenming;YU Miao;MA Xianyong;YE Xiufeng(Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation,Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition,Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture and Rural Affairs,State Key Laboratory of Livestock and Poultry Breeding,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Guangdong Laboratory for Lingnan Modern Agriculture,Maoming Branch,Maoming 525000,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2023年第8期4988-4997,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
广东省乡村振兴战略专项资金(2023TS-3,2023KJ465)
广东省饲料产业技术体系创新团队建设项目(2023KJ115)
广东省农业科学院学科团队建设项目(202118TD)
科技创新战略专项资金(高水平农科院建设)——杰出人才(R2020PY-JC001)和优秀博士(R2020YJ-YB2002)
广州市民生科技项目(202002020077)。
关键词
纳米氧化锌
仔猪
抗氧化能力
游离氨基酸
nano zinc oxide
piglets
antioxidant capacity
free amino acid
作者简介
崔艺燕(1987—),女,广州佛山人,助理研究员,硕士,从事动物营养与饲料科学、生态养殖与环境控制研究。E⁃mail:958117076@qq.com;通信作者:叶秀峰,高级畜牧师,E⁃mail:Y13011@126.com。
