摘要
目的优化超声提取高良姜中高良姜素的工艺条件并测定高良姜素的抗氧化活性。方法采用高效液相色谱法对高良姜素进行定量分析。以高良姜醇提液中高良姜素的含量为指标,通过单因素试验考察乙醇浓度、提取时间以及高良姜与乙醇的料液比对高良姜素含量的影响,通过正交试验筛选最佳提取工艺条件。抗氧化活性实验进行DPPH和ABTS自由基清除能力测定及铁离子还原能力测定。结果最优提取工艺为1:35料液比,70%乙醇浓度,超声提取1 h。在此条件下进行提取,高良姜素平均含量为1.06%,相对标准偏差为1.30%。在0.05~0.5 g/L浓度内,高良姜素对DPPH、ABTS的清除率及对铁离子的还原能力均随着浓度的增加而升高。结论优化高良姜素提取工艺简单可行;高良姜素具有一定的抗氧化活性。
Objective To optimize the process conditions for the extraction of galangin from galangal by ultrasound and to determine the antioxidant activity of galangin.Methods High performance liquid chromatography(HPLC)was used for the quantitative analysis of galangin.With the content of galangin in Galangal alcohol extract as an indicator,the effects of ethanol concentration,extraction time and the solid-to-liquid ratio of galangal to ethanol on the content of galangin were investigated by single-factor tests,and the optimum extraction process conditions were screened by orthogonal tests.Antioxidant activity assay was carried out to determine the DPPH and ABTS radical scavenging capacity as well as the ferric ion reducing capacity.Results The optimal extraction process conditions included a 1:35 stock-to-liquid ratio,70% ethanol concentration and 1 h ultrasonic extraction,under which the extraction could achieve a mean galangin content of 1.06% with a relative standard deviation of 1.30%.The scavenging capacity of galangin for DPPH and ABTS radicals and the ferric ion reducing capacity of galangin increased with the increase of concentration.Conclusion The optimization of galangin extraction process is simple and feasible;Galangin has certain antioxidant activity.
作者
冯真英
李泽森
陈丹
彭素绢
李观同
孙玉香
周中流
FENG Zhen-ying;LI Ze-seng;CHEN Dan;PENG Su-juan;LI Guan-tong;SUN Yu-xiang;ZHOU Zhong-liu(The Second Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China;Lingnan Normal University,Zhanjiang 524048,China)
出处
《广东医科大学学报》
2023年第4期373-377,382,共6页
Journal of Guangdong Medical University
基金
湛江市科技计划项目(2022A01111)
广东省重点学科科研项目(2019-GDXK-0025、2021ZDJS035)。
关键词
高良姜素
超声提取
单因素试验
正交试验
抗氧化
galangin
ultrasonic extraction
single-factor test
orthogonal test
antioxidant
作者简介
冯真英(1980-),女,本科,副主任药师,E-mail:846935821@qq.com;通信作者:周中流,博士,教授,E-mail:zhou110zhong99@sohu.com。
