摘要
本研究对从蜘蛛香(Valeriana jatamansi Jones)和赤芍(Paeoniae Rubra Radix)中分离的一种苯丙素类化合物ferulaldehyde的抗甲型流感病毒的作用机制进行探索。通过加药时间点、免疫荧光实验、红细胞凝集法与神经氨酸酶活力检测等实验,发现化合物ferulaldehyde主要影响流感病毒的复制和释放环节,干扰病毒的生命周期从而发挥抗流感病毒活性。分子对接结果进一步确认流感病毒NP和NA蛋白很可能是它作用的靶点。同时,该化合物还能显著抑制由流感病毒引起的巨噬细胞炎症相关蛋白和趋化因子的表达。综上所述,化合物ferulaldehyde可能通过直接抑制流感病毒复制和释放的过程以及影响免疫细胞的功能从而发挥抗流感的作用。
To investigates the mechanism of ferulaldehyde,a phenylpropanoid compound isolated from Valeriana jatamansi Jones and Paeoniae Rubra Radix,against influenza A virus.Through experiments such as dosing time point,immunofluorescence,red blood cell agglutination,and neuraminidase activity detection,it was found that feruladehyde mainly affected the replication and release of influenza virus,interfering with the life cycle of the virus,thereby exerting its anti-influenza virus activity.Molecular docking simulation confirmed that NP and NA might be the targets of the compound anti-influenza virus.In addition,the compound significantly inhibited the expression of macrophage inflammation-associated proteins iNOS and COX-2 and chemokine IP-10 induced by influenza virus.In conclusion,the compound ferulaldehyde might exert anti-influenza effects by directly inhibiting influenza virus replication and release and by affecting the function of immune cells.
作者
周艳
宿露
王云
范韦佟
李洪梅
李蓉涛
刘丹
ZHOU Yan;SU Lu;WANG Yun;FAN Wei-tong;LI Hong-mei;LI Rong-tao;LIU Dan(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第8期1364-1371,共8页
Natural Product Research and Development
基金
国家自然科学基金(82204248)。
作者简介
通信作者:刘丹,E-mail:liudankm512@163.com。
