摘要
长链非编码RNA(lncRNA)通常认为不具有蛋白质编码能力,广泛参与细胞增殖、信号转导等生命过程。但近年来研究表明,部分lncRNA的短开放阅读框(sORF)可编码具有生物活性的短于100个氨基酸的小肽,在钙离子稳态、胚胎发育等生命活动及恶性肿瘤的发生发展中发挥作用,是潜在的药物靶点和癌症生物标志物。目前大多采用生物信息学工具预测可编码的sORF、核糖体图谱筛选潜在编码的lncRNA、体外翻译初步检测lncRNA的编码能力、质谱鉴定潜在翻译的小肽、特异性抗体及表位标签验证小肽的内源性表达等。本文介绍了近年来鉴定的lncRNA编码小肽的生理功能、病理作用以及预测lncRNA编码潜力的研究策略。
Long non-coding RNAs(lncRNAs)which are usually thought to have no protein coding ability,are widely involved in cell proliferation,signal transduction and other biological activities.However,recent studies have suggested that short open reading frames(sORFs)of some lncRNAs can encode small functional peptides(micropeptides).These micropeptides appear to play important roles in calcium homeostasis,embryonic development and tumorigenesis,suggesting their potential as therapeutic targets and diagnostic biomarkers.Currently,bioinformatic tools as well as experimental methods such as ribosome mapping and in vitro translation are applied to predict the coding potential of lncRNAs.Furthermore,mass spectrometry,specific antibodies and epitope tags are used for validating the expression of micropeptides.Here,we review the physiological and pathological functions of recently identified micropeptides as well as research strategies for predicting the coding potential of lncRNAs to facilitate the further research of lncRNA encoded micropeptides.
作者
王心怡
张祯
石成瑜
王颖
周天华
林爱福
WANG Xinyi;ZHANG Zhen;SHI Chengyu;WANG Ying;ZHOU Tianhua;LIN Aifu(College of Life Sciences,Zhejiang University,Hangzhou 310058,China;Zhejiang University Cancer Center,Hangzhou 310058,China;The Fourth Affiliated Hospital,Zhejiang University School of Medicine,Center for RNA Medicine,International Institutes of Medicine,Zhejiang University,Jinhua 322000,Zhejiang Province,China;Department of Cell Biology,Zhejiang University School of Medicine,Hangzhou 310058,China)
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2023年第4期397-405,共9页
Journal of Zhejiang University(Medical Sciences)
基金
国家重点研发计划(2021YFC2700903)
国家杰出青年科学基金(32225014)
浙江省“领雁”研发攻关计划(2023C03023)。
关键词
长链非编码RNA
短开放阅读框
小肽
核糖体图谱
质谱
综述
Long non-coding RNA
Short open reading frame
Micropeptide
Ribosome profiling
Mass spectrometry
Review
作者简介
第一作者:王心怡,硕士研究生,主要从事lncRNA编码小肽生物功能及机制研究,E-mail:22207016@zju.edu.cn,https://orcid.org/0009-0000-3673-5250;通信作者:林爱福,教授,博士生导师,主要从事分子细胞生物学基础研究、肿瘤免疫与靶向转化研究,E-mail:linaifu@zju.edu.cn,https://orcid.org/0000-0002-3968-3617;通信作者:周天华,教授,博士生导师,主要从事分子细胞生物学相关研究,E-mail:tzhou@zju.edu.cn,https://orcid.org/0000-0002-1791-2124。
