摘要
上转换纳米粒子(UCPs)近红外光激发的特性不仅可有效避免复杂生物样本的背景干扰,还可以克服散射光和纸张添加剂的干扰。本文尝试将UCPs用于纸基分析装置,构建了以UCPs作为能量供体,以氧化碳球(CNPs)为能量受体基于荧光共振能量转移(FRET)的纸芯片,利用核酸适配体与目标物的特异性识别作用,实现了Ag^(+)的纸上检测。向纸芯片检测区加入CNPs后,CNPs与UCNPs表面的Ag^(+)适配体通过π-π堆积作用组装在一起,发生FRET过程,使UCNPs荧光猝灭;加入Ag^(+)后,Ag^(+)与适配体特异性结合,FRET消失,UC-NPs荧光恢复,荧光恢复程度随着Ag^(+)浓度的增大而增大。该纸芯片对Ag^(+)表现出良好的特异性,检测线性范围为0.05~50 ng·mL^(-1),线性相关系数为0.9901,检测限为0.01 ng·mL^(-1)。
UCPs can not only effectively avoid the background interference of complex biological samples,but also overcome the interference of scattered light and paper additives.We attempted to apply UCPs to paper-based analysis device.A paper chip based on fluorescence resonance energy transfer(FRET)was constructed with upconversion fluorescence nanomaterials(UCPs)as energy donors and carbon oxide spheres as energy acceptors.The Ag^(+)was detected on paper by nucleic acid aptamer and target specific recognition.When CNPs was added to the detection area of the paper chip,the Ag^(+)aptamer and UCNPs were assembled together,and a FRET process occurred,which caused the fluorescence quenching of UCNPs.After the addition of Ag^(+),Ag^(+)specifically bound to the aptamer,FRET disappeared,and the fluorescence of UCNPs resumed,and the degree of fluorescence recovery increased with the concentration of Ag^(+).The paper chip showed good specificity for Ag^(+),the detection linear range was 0.05~50 ng·mL^(-1),the linear correlation coefficient was 0.9901,and the detection limit was 0.01 ng·mL^(-1).
作者
李田霞
陈峰
LI Tian-xia;CHEN Feng(School of Biological Engineering,Wuhan Polytechnic,Wuhan 430074;School of Mechanical Engineering,Wuhan Polytechnic,Wuhan 430074)
出处
《化学研究与应用》
CAS
北大核心
2023年第8期1945-1949,共5页
Chemical Research and Application
基金
湖北省教育厅研究项目(B2020380)资助
武汉职业技术学院研究项目(2021YK009)资助。
关键词
纸芯片
荧光共振能量转移
适配体
银离子
paper chip
fluorescence resonance energy transfer
aptamer
Ag^(+)
作者简介
联系人:李田霞(1981-),女,副教授,主要从事生物传感器研究。E-mail:406571573@qq.com。
