摘要
牛乳铁素(Bovine lactoferricin,Lfcin B)是牛乳铁蛋白经胰酶消化后释放出的17位至41位氨基酸残基构成的短肽,具有广谱抗菌、抗病毒、抗肿瘤和抗寄生虫等生物活性。为了深入开发和利用Lfcin B,进一步去除Lfcin B的部分C-末端氨基酸残基,形成了由10个氨基酸残基构成的短肽,称为Lfcin B_(1-10)。Lfcin B1-10较好地保留了Lfcin B的生物活性,但有关Lfcin B_(1-10)的理化性质、结构、跨膜区等是否改变尚不清楚。因此,利用生物信息学方法对Lfcin B_(1-10)的氨基酸的理化性质、二级结构、跨膜区、信号肽及酶切位点等特征进行预测分析。结果显示,Lfcin B_(1-10)为阳离子型抗菌肽,理论等电点为11.71,热稳定性较差;分子为亲水性寡肽,二级结构仅有无规则卷曲,无跨膜区和信号肽,可被机体内多种酶降解,具有良好安全性。研究不仅为深入认识其构效关系、抗菌机制提供依据,同时为Lfcin B1-10的实际应用提供参考。
A short peptide was composed of amino acid residues at positions 17 to 41 released from bovine lactoferrin(Lfcin B)digested by pancreatin.The short peptide had broad-spectrum antibacterial,antiviral,antitumor and antiparasitic activities.In order to further develop Lfcin B,a short peptide composed of 10 amino acid residues was obtained from C-terminal amino acid in Lfcin B.The short peptide was named Lfcin B_(1-10).Lfcin B_(1-10) retained the biological activity of Lfcin B well,but it was not clear that whether the physicochemical properties,structure and transmembrane region of Lfcin B_(1-10) had changed.Bioinformatics methods were used to predict the characteristics of amino acids in Lfcin B_(1-10),such as physicochemical properties,secondary structure,transmembrane region,signal peptide and restriction site.The results showed that Lfcin B_(1-10) was a cationic antibacterial peptide with a theoretical isoelectric point of 11.71 and poor thermal stability.The molecule is a hydrophilic oligopeptide,and its secondary structure had only irregular curl,no transmembrane region and signal peptide.It could be degraded by various enzymes in the body,and it had good safety,which provided a basis for understanding the structure-activity relationship and antibacterial mechanism of Lfcin B_(1-10),but also provided a reference for the practical application of Lfcin B_(1-10).
作者
宋成祺
于立权
崔玉东
Song Chengqi;Yu Liquan;Cui Yudong(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing163319;College of Life Science and Technology,Heilongjiang Bayi Agricultural University)
出处
《黑龙江八一农垦大学学报》
2023年第4期81-86,104,共7页
journal of heilongjiang bayi agricultural university
基金
博士后研究人员落户黑龙江科研启动项目(LBH-Q15113)
黑龙江八一农垦大学培育课题重点项目(XA2015-05)。
作者简介
宋成祺(1993-),男,黑龙江八一农垦大学动物科技学院2018级硕士研究生;通信作者:于立权,男,讲师,E-mail:flyylq@163.com;通信作者:崔玉东,男,教授,博士研究生导师,E-mail:ydcui4053@163.com。
