摘要
目的基于16SrDNA高通量测序探究HPV52感染对女性阴道微生态的影响。方法选取2022年4~12月长沙市第一医院妇科、九江市第三人民医院妇科收治的女性患者142例,进行阴道菌群检测和HPV52检测,分析阴道菌群特征与HPV52感染之间的关系。结果共检出阴道菌门28个,阴道菌属453个,其中主要菌门为厚壁菌门,其它依次为放线菌门、拟杆菌门等。主要菌属为乳杆菌属,其它依次为加德纳菌属、嗜胨菌属等。经测序共获得8689个OTU,其中HPV52感染组特有1768个,HPV阴性组特有2114个,2组共有4807个。HPV52阳性组ACE指数、Shannon指数以及Chaol指数均稍高于HPV52阴性组,Simpson指数稍低于HPV52阴性组。门水平以及属水平HPV52阳性患者与HPV52阴性患者菌群分布存在一定差异,网络图直观反应HPV52阳性患者与HPV52阴性患者阴道菌群分布情况。结论HPV52阳性以及HPV52阴性患者主要感染菌门均为厚壁菌门,均以乳杆菌属为优势菌,HPV52阳性组患者阴道菌群多样性高于HPV52阴性患者,HPV52感染可能与嗜胨菌属、纤毛菌属等水平升高有关,因此可从维持阴道菌群平衡角度预防和治疗HPV52感染所致的相关疾病。
Objective Exploring the impact of HPV52 infection on female vaginal microbiota based on high-throughput 16SrDNA sequencing.Methods From April 2022 to December 2022,142 female patients admitted to the gynecology department of Changsha First Hospital and the gynecology department of Jiujiang Third People's Hospital were selected for Vaginal flora detection and HPV52 detection,and the relationship between the characteristics of Vaginal flora and HPV52 infection was analyzed.Results A total of 28 vaginal bacteria and 453 vaginal bacteria were detected,of which the main bacteria was Bacillota,and the others were Actinomycetota,Bacteroidota,etc.The main bacteria are Lactobacillus,followed by Gardner and Peptoniphilus.After sequencing,a total of 8689 OTUs were obtained,including 1768 unique to the HPV52 infection group,2114 unique to the HPV negative group,and 4807 unique to the two groups.The ACE index,Shannon index,and Chaol index of the HPV52 positive group were slightly higher than those of the HPV52 negative group,while the Simpson index was slightly lower than those of the HPV52 negative group.There is a certain difference in the distribution of flora between HPV52 positive patients and HPV52 negative patients at the gate level and genus level.The network diagram directly reflects the distribution of Vaginal flora between HPV52 positive patients and HPV52 negative patients.Conclusion The main infectious bacteria of HPV52 positive and HPV52 negative patients are Bacillota,and Lactobacillus is the dominant bacteria.The diversity of Vaginal flora of HPV52 positive patients is higher than that of HPV52 negative patients.HPV52 infection may be related to the increase of Peptoniphilus,ciliates and other levels.Therefore,related diseases caused by HPV52 infection can be prevented and treated from the perspective of maintaining the balance of Vaginal flora.
作者
祝江红
黄蕾
韦妹艳
ZHU Jianghong;HUANG Lei;WEI Meiyan(Department of Gynecology,The Third People's Hospital of Jiujiang Jiangi 332000,China;Department of Gynecology,The First Hospital of Changsha;Pathological Laboratory,TheFirst Hospital of Changsha)
出处
《中国病原生物学杂志》
CSCD
北大核心
2023年第9期1005-1011,共7页
Journal of Pathogen Biology
基金
长沙市自然科学基金项目(No.kq2202004)。
关键词
阴道菌群
人乳头瘤病毒52
微生态
高通量测序
vaginal microbiota
human papllomavirus 52
microecology
high-throughput sequencing
作者简介
通讯作者:黄蕾,E-mail:huanglei696969@163.com;祝江红(1986-),女,江西樟树人,主治医师,硕士研究生。主要研究方向:妇科。E-mail:1484859702@qq.com
