摘要
当前,大多数体内应用所使用的光源为具有光毒性和低组织穿透能力的紫外(UV)或可见光,这就使得可利用近红外光(NIR)激活的上转换纳米粒子(UCNPs)平台受到越来越多的关注,这是因为UCNPs所吸收的NIR激发光在780~2500nm之间,生物组织对该波长范围的光吸收很低,造成NIR的组织穿透力明显强于UV和可见光,又因NIR能量较低,对生物组织的伤害也比较小,这些优势促使充当换能器的UCNPs在成像、光动力疗法(PDT)、光遗传学和光控药物/生物分子释放等诸多领域取得了广泛应用。本实验综合采用微乳液法制备UCNPs组装体,使用多种技术对材料的结构和性能进行表征,能让学生明白自组装的原理,学习自组装的制备工艺,掌握粒径仪、光谱仪、扫描电镜和透射电镜的基本操作,较为系统地完成材料制备、结构表征和性能测试三个过程的综合训练。
Currently,most of the light sources used for in vivo applications are ultraviolet(UV)or visible light with phototoxicity and low tissue penetration,which enables the near-infrared(NIR)-activated upconversion nanoparticles(UCNPs)platforms to receive more and more attention,because the NIR light absorbed by UCNPs is between 780~2500 nm,and the light absorption of biological tissues in this wavelength range is very low,resulting in the tissue penetration of NIR being significantly stronger than that of UV and visible light.On the other hand,due to the low energy of NIR,it has relatively little damage to biological tissues.All the above advantages promote UCNPs as good nano-transducers in imaging,photodynamic therapy(PDT),optogenetics,light-controlled drug/biomolecular release,and many other applied fields.In this experiment,the micro-emulsion method was chosen to prepare UCNPs assemblies,and various techniques were used to characterize the structure and properties of the materials.This experiment allows students to understand the principle of self-assembly,learn the preparation technology of self-assembly,and master the basic operations of particle size analyzer,spectrometer,scanning electron microscope and transmission electron microscopy.This experiment will help students systematically complete the comprehensive training of the three processes of material preparation,structure characterization,and performance testing.
作者
张振
Zhang Zhen(School of Materials Science and Engineering,Sun Yat-sen University,Guangdong,510006)
出处
《当代化工研究》
CAS
2023年第17期35-38,共4页
Modern Chemical Research
基金
2022年国家自然科学基金(青年)项目“聚合物与上转换纳米粒子复合物组装体的构筑及其程序化光激活疗法研究”(项目编号:52103276)
2022年广东省自然科学基金面上项目“正交发射光上转换纳米颗粒组装体的制备与应用研究”(项目编号:2022A1515010947)
2023年中央高校基本科研业务费-青年教师培育项目“磁靶向上转换基多功能复合粒子的制备及其相关生物应用探究”(项目编号:23qnpy03)
2023年中山大学教学质量与教学改革类项目(项目编号:29000-12220011)。
关键词
上转换
微乳液
自组装
结构表征
upconversion
micro-emulsion
self-assembly
structure characterization
作者简介
张振(1990-),男,汉族,河南洛阳人,博士研究生,副教授,研究方向:高分子和上转换纳米材料合成、组装及其相关生物应用。
