摘要
本文以杏鲍菇废弃菌渣为原料,探究了D-氨基葡萄糖盐酸盐(D-glucosamine hydrochloride,GAH)的制备工艺、液相-质谱(HPLC-MS)、红外光谱、理化指标及其对斑马鱼胚胎发育的影响。采用单因素和响应面优化试验,获得盐酸水解制备GAH的最佳条件:盐酸浓度31%,水解时间4 h,水解温度82℃,液固比5 mL/g,此时GAH得率可达23.61%。液相-质谱、红外光谱和理化指标分析显示,GAH纯化样品纯度是标准品的101.9%,质谱和红外光谱图与标准品一致,各项指标均符合甚至优于美国药典43-国家处方集38(USP43-NF38)的质量标准,砷含量仅0.21μg/g。斑马鱼实验结果表明,中高浓度(0.3%~0.5%)的GAH引发的斑马鱼胚胎发育异常比例超过50%,且0.3%的GAH对转化生长因子-β信号通路具有明显的促进作用。本研究结果可为未来杏鲍菇产品产业化生产及应用提供参考。
In this paper,the preparation process of D-glucosamine hydrochloride(GAH)was investigated from the waste residues of Pleurotus eryngii(P.eryngii).Liquid chromatography-mass spectrometry(HPLC-MS),infrared spectroscopy(IR),and physicochemical indexes of GAH were also studied.Then the effect of GAH on the zebrafish embryonic development was evaluated.The experimental conditions for preparing GAH were optimized through single-factor experiments and response surface methodology.Under optimal conditions(HCl concentration of 31%,hydrolysis time of 4 h,hydrolysis temperature of 82℃,and the liquid-solid ratio of 5 mL/g),the yield of GAH reached 23.61%.HPLC-MS,IR,and physicochemical index analysis showed that the purity of the GAH-purified sample was 101.9%of the standard.The mass spectrometry and infrared spectroscopy were consistent with the standard.All indexes met or even outperformed the specifications of the United States Pharmacopeia 43-National Formulary 38(USP 43-NF38),and the arsenic content was only 0.21μg/g.In addition,the zebrafish experiment indicated that exposure to moderate and high concentrations(0.3%~0.5%)of GAH could induce more than 50%of the abnormal embryonic development of zebrafish.Moreover,0.3%of GAH promotes the transforming growth factor-β(TGF-β)signal pathway.In conclusion,the results provide valuable insight into preparing and applying the industrial residue of P.eryngii.
作者
张倩如
吴启赐
薛钰
林志超
黄家福
吕昊坤
彭伟
潘裕添
林进妹
ZHANG Qianru;WU Qici;XUE Yu;LIN Zhichao;HUANG Jiafu;LÜHaokun;PENG Wei;PAN Yutian;LIN Jinmei(Fujian Universities Engineering Research Center of Fungus Industry,Minnan Normal University,Zhangzhou 363000,China;College of Chemistry and Environment,Minnan Normal University,Zhangzhou 363000,China)
出处
《食品工业科技》
CAS
北大核心
2023年第17期263-271,共9页
Science and Technology of Food Industry
基金
福建省自然科学基金面上项目(2020J01822,2020J01823)
国家科技项目备案(2021L3027)
福建省教育厅中青年教师教育科研科技类项目(JT180306)
闽南师范大学培育项目(MSPY202101)
福建省科技厅对外合作项目(2022I0022)。
关键词
D-氨基葡萄糖盐酸盐
杏鲍菇
废弃菌渣
响应面优化
斑马鱼胚胎发育
D-glucosamine hydrochloride
Pleurotus eryngii
waste residues
response surface optimization
zebrafish embryonic development
作者简介
张倩如(1997-),女,硕士研究生,研究方向:化学生物学,E-mail:zq20110823@163.com;通信作者:吴启赐(1984-),男,博士,讲师,研究方向:天然产物化学,E-mail:wuqiyang168@163.com;林进妹(1969-),女,本科,副教授,研究方向:天然产物化学,E-mail:ljm0542@mnnu.edu.cn。
