摘要
药学综合实验教学是提高学生创新实践能力的重要途径,采用Plackett-Burman、正交实验设计法开展阿霉素白蛋白微球制备的药学综合实验,进行了微球处方的筛选和优化。通过显微镜观察微球形态,分光光度计法测定阿霉素的含量及包封率,动态透析法考察体外药物释放情况。该综合实验立足于药物高端制剂开发技术,将科研与教学相结合,不但使学生掌握微球制备、实验设计方法、药物分析和性质表征等专业综合知识,而且有利于培养学生科研思路,激发研究兴趣,提高知识运用和创新实践能力。
Comprehensive experiment is an important way in improving students' innovation and practical ability of pharmacy.Design of experiments is a common scientific and efficient research method in pharmaceutical study.Herein,a comprehensive experiment is designed for preparation of doxorubicin loaded albumin microspheres by the Plackett-Burman and orthogonal design methods,which are used to screen and optimize the formulations of the microspheres.The morphology,drug content and encapsulation rate of the microspheres are respectively determined by microscope and spectrophotometer,and in vitro drug release is investigated by dynamic dialysis.This comprehensive experiment of innovative formulation combines scientific research with teaching,which not only enables students to master the comprehensive professional knowledge of microsphere preparations,experiment design methods,drug analysis and characterizations,but also facilitates to cultivate students' scientific ideas,stimulate study interests,and improve their knowledge application and innovation ability.
作者
邱立朋
胡笳
方文杰
陈敬华
QIU Lipeng;HU Jia;FANG Wenjie;CHEN Jinghua(School of Life Sciences and Health Engineering,Jiangnan University,Wuxi 214122,Jiangsu,China)
出处
《实验室研究与探索》
CAS
北大核心
2023年第5期238-242,共5页
Research and Exploration In Laboratory
基金
江苏省品牌专业建设工程项目(PPZY2015B146)
江南大学本科教育教学改革研究重点项目(JG2021002)
江南大学本科教育教学改革研究项目资助(JG2021006)。
关键词
微球
药学综合实验
实验设计
创新实践
microsphere
comprehensive experiment in pharmacy
design of experiment
innovative practice
作者简介
邱立朋(1984-),男,山东临沂人,博士,副教授,主要从事药物创新制剂研究。Tel.:0510-85329042,E-mail:qiulp@jiangnan.edu.cn。
