摘要
Background and Aims:Hepatocellular carcinoma(HCC)is among the most common malignant tumors globally.Circular RNAs(circRNAs),as a type of noncoding RNAs,reportedly participate in various tumor biological processes.However,the role of circHDAC1_004 in HCC remains unclear.Thus,we aimed to explore the role and the underlying mechanisms of circHDAC1_004 in the development and progression of HCC.Methods:Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect circHDAC1_004 expression(circ_0005339)in HCC.Sanger sequencing and agarose gel electrophoresis were used to determine the structure of circHDAC1_004.In vitro and in vivo experiments were used to determine the biological function of circHDAC1_004 in HCC.Herein,qRT-PCR,RNA immunoprecipitation,western blotting,and a luciferase reporter assay were used to explore the relationships among circHDAC1_004,miR-361-3p,and NACC1.Results:circHDAC1_004 was upregulated in HCC and significantly associated with poor overall survival.circHDAC1_004 promoted HCC cell proliferation,stemness,migration,and invasion.In addition,circHDAC1_004 upregulated human umbilical vein endothelial cells(HUVECs)and promoted angiogenesis through exosomes.circHDAC1_004 promoted NACC1 expression and stimulated the epithelialmesenchymal transition pathway by sponging miR-361-3p.Conclusions:We found that circHDAC1_004 overexpression enhanced the proliferation,stemness,and metastasis of HCC via the miR-361-3p/NACC1 axis and promoted HCC angiogenesis through exosomes.Our findings may help develop a possible therapeutic strategy for HCC.
基金
supported by the National Natural Science Foundation of China(grant number 81871260)
The training programe of“Double hundred”young and middle-aged medical and health talents in Wuxi(grant number BJ020034)
Health Research Projects of Wuxi Health Committee(grant number M202106)。
作者简介
Correspondence to:Lianbao Kong,Hepatobiliary Center,The First Affiliated Hospital of Nanjing Medical University,300 Guangzhou Road,Nanjing,Jiangsu 210029,China.ORCID:https://orcid.org/0000-0003-2508-1321.Tel:+86-13801583199,Fax:+86-25-68303221,E-mail:lbkong@njmu.edu.cn;Yongping Zhou,Department of Hepatobiliary Surgery,Jiangnan University Medical Center,JUMC,No.68 Zhongshan Road,Wuxi,Jiangsu 214000,China.Tel:+86-15052115599,Fax:+86-510-68562571,E-mail:zyp19840527@njmu.edu.cn。
