摘要
目的 探讨程序性死亡受体1(programmed death receptor 1,PD-1)基因单核苷酸多态性与肝细胞癌血液学分子标志物的关联性研究。方法 选择2021年1-12月南通大学附属医院介入放射科收治的165例新发肝癌患者作为入组对象,提取基因组DNA,采用Panel高通量数据分析进行PD-1基因分型,分析各基因型与肝癌血液学分子标志物的关联性。结果 AFP的不同分组情况,在rs10204525、rs2227982、rs36084323基因分型情况,各位点突变基因型在AFP不同分组上差异无统计学意义(P>0.05);PIVKA-Ⅱ不同分组上,对于rs2227982分组,野生型CC、杂合突变型CT、纯合突变型TT、突变型CT+TT在PIVKA-Ⅱ(-)与PIVKA-Ⅱ(+)比较,差异无统计学意义(P> 0.05),而对rs2227982分组,其中杂合突变型AG(34例vs19例)、纯合突变型AA在PIVKA-Ⅱ(-)与PIVKA-Ⅱ(+)(30例vs15例)比较差异有统计学意义(χ^(2)=3.125,7.158P<0.05);rs10204525、rs36084323分组,CT、TT、CT+TT与PIVKA-Ⅱ指标无明显关联(P>0.05)。结论 PD-1基因rs36084323位点单核苷酸多态性与异常凝血酶原指标之间存在一定关联。
Objective To explore the correlation between Programmed death receptor 1(PD-1)gene single nucleotide polymorphisms and hematologic molecular markers of hepatocellular carcinoma.Methods A total of 165 patients with newly diagnosed liver cancer admitted to the Department of Interventional Radiology,Affiliated Hospital of Nantong University between 2021-01-01 and 2021-12-31 were selected as enrolled subjects.Genomic DNA was extracted and PD-1 genotyping was performed by using panel high-throughput data analysis,and the association between each genotype and hematologic molecular markers of liver cancer was analyzed.Results There was no significant difference in AFP genotyping among different AFP groups,including rs10204525,rs2227982 and rs36084323(P>0.05).In different PIVKA-Ⅱgroups,for rs2227982 group,wild-type CC,heterozygous mutant CT,homozygous mutant TT and mutant CT+TT showed no statistical significance(P>0.05),but for rs2227982 group,There were significant differences between heterozygous mutant AG(34 vs19 cases)and homozygous mutant AA in PIVKA-Ⅱ(-)and PIVKA-Ⅱ(+)(30 vs15 cases)(χ^(2)=3.125,7.158P<0.05).In rs10204525 and rs36084323 groups,CT,TT,CT+TT had no significant correlation with PIVKA-Ⅱindexes(P>0.05).Conclusion PD-1 gene rs36084323 single nucleotide polymorphism is associated with abnormal prothrombin index.
作者
陆小华
袁洪新
LU Xiaohua;YUAN Hongxin(Affiliated Hospital of Nantong University,Interventional radiology department,Nantong,Jiangsu 226001)
出处
《智慧健康》
2023年第15期97-101,共5页
Smart Healthcare
基金
江苏省南通市科技局2021基础科学研究公众健康项目《负性T细胞共刺激途径关键基因单核苷酸多态性与南通地区汉族人群肝癌TACE疗效及预后的关联研究》(项目编号:JC2021196)。
关键词
PD-1基因
单核苷酸多态性
肝细胞癌
血液分子标志物
相关性
PD-1 gene
Single nucleotide polymorphism
Hepatocellular carcinoma
Blood molecular markers
Correlation
作者简介
陆小华(1982-),男,江苏南通,副主任医师,硕士,研究方向:介入放射科。
