摘要
以鸭瘦素受体(LEPR)基因为研究对象,运用生物信息学方法对鸭等10种动物的LEPR蛋白进行多序列比对分析、分子进化分析及蛋白质结构分析,并预测LEPR蛋白的理化性质、疏水性等基本性质。结果表明:鸭LEPR蛋白预测为不稳定的亲水性蛋白质,不含信号肽,为非分泌型蛋白质,定位在细胞质膜上,与其生物学功能相符。鸭LEPR蛋白还含有132个潜在磷酸化位点、10个N型糖基化位点和6个O型糖基化修饰位点。进化关系分析表明,鸭LEPR基因与同为水禽的黑天鹅进化关系最近,具有较高的同源性。这一研究为进一步研究鸭LEPR基因及其编码的蛋白质结构和功能提供了参考。
In this study,the sequence alignment,molecular evolution and protein structure of LEPR protein from 1o kinds of animalswere analyzed using bioinformatics analysis.The physical,chemical properties and hydrophobicity of LEPR protein were also predicted.The results showed that duck LEPR protein was predicted to be an unstable hydrophilic protein,without signal peptide,non-secreted protein,and located in the plasma membrane,which was consistent with its biological function.In addition,the protein contained 132 potential phosphorylation sites,1o N-type glycosylation sites and 6 O-type glycosylation modification sites.Evolutionary relationship analysis showed that duck LEPR gene was closely related to that of black swan,and which had high homology.The predicted results of the present study will provide the reference for further studying the LEPR gene,structure and function of its coding protein.
作者
税斐
邱桂如
江洪峰
郑书丽
夏晶晶
贾羽晴
郭立平
陈兴勇
耿照玉
金四华
SHUI Fei;QIU Guiru;JIANG Hongfeng;ZHENG Shuli;XIA Jingjing;JIA Yuqing;GUO Liping;CHEN Xingyong;GENG Zhaoyu;JIN Sihua(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,Chins;School of Public Health,Anhui Medical University,Hefei 230032,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2023年第3期11-17,36,共8页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
安徽省家禽产业技术体系专项(AHCYJSTX-06)
安徽省科技重大专项(201903a06020018)
安徽省高校协同创新项目(GXXT-2021-052)
安徽省科技研发项目(KJ2021040)。
作者简介
税斐(1997-),女,四川攀枝花人,安徽农业大学硕士研究生,主要从事家禽分子遗传育种研究;通信作者:金四华,安徽农业大学副教授、硕导,主要从事家禽遗传育种研究,E-mail:jsh3235@126.com。
