摘要
为了研究应用育种群体中的剩余遗传变异开展QTL定位的可行性,本研究利用高代育种株系‘大粒香’/‘Mizuhotigara’中的剩余遗传变异,采用QTL-seq对水稻抽穗期QTL进行检测,利用连锁分析对检测到的QTL进行验证。育种组合‘大粒香’/‘Mizuhotigara’的F5选种圃中的株系G1140的抽穗期表现为连续双峰分布,推测该株系内可能存在主效抽穗期基因的等位变异。从株系G1140中挑选4个单株分别自交得到4套F6群体,其中群体H2的抽穗期表现为连续的双峰分布。利用H2群体中抽穗极早和极晚单株的DNA分别构建DNA混池,采用QTL-seq进行抽穗期QTL定位,在第5染色体0~4.95 Mb和第10染色体16.64~18.27 Mb区间各检测到1个QTL,分别命名为qHd5和qHd10。利用H2群体采用连锁分析法对qHd5和qHd10进行验证,q Hd5的加性效应为2.3 d,增效等位基因来自‘大粒香’,贡献率为4.2%;qHd10的加性效应为3.3 d,增效等位基因来自‘Mizuhotigara’,贡献率为10.7%。本研究结果为作物QTL定位提供了新的思路。
To study the possibility of QTL mapping which applies residual genetic variation in breeding populations.This study detected rice heading date QTL by application of residual genetic variation from advanced breeding line of Dalixiang/Mizuhotigara.QTL-seq was applied for QTL analysis,and the detected QTLs were validated by linkage analysis.Line G1140 resulted from cross of Dalixiang/Mizuhotigara in F5 selective nursery showed continuous bimodal distribution for heading date.It could be speculated that there is major-effect allelic variation for heading date in G1140.Selfing seeds of four plants selected from G1140 resulted in four F6 populations,among which the population H2 exhibited continuous bimodal distribution for heading date.Bulked DNA pools were constructed using the DNA of extremely early and late plants in H2.QTL-seq was applied for heading date QTL analysis.Two QTLs were detected in the regions 0~4.95 Mb on chromosome 5 and 16.64~18.27 Mb on chromosome 10,and were named as qHd5 and qHd10.The two QTLs were validated by linkage analysis using H2 population.qHd5 explained 4.2%of the phenotypic variation,with the Dalixiang allele delaying heading by 2.3 d.qHd10 explained 10.7%of the phenotypic variation,with the Mizuhotigara allele delaying heading by 3.3 d.The results of this study provide new idea for crop QTL mapping.
作者
王凌志
张宏伟
谢英
郝留根
王珍珍
易崇粉
郭慧
甘雨
向关伦
杨占烈
Wang Lingzhi;Zhang Hongwei;Xie Ying;Hao Liugen;Wang Zhenzhen;Yi Chongfen;Guo Hui;Gan Yu;Xiang Guanlun;Yang Zhanlie(College of Agriculture,Guizhou University,Guiyang,550002;Rice Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang,550006;Qiandongnan Academy of Agricultural Sciences,Kaili,556000;College of Life Sciences,Guizhou University,Guiyang,550025)
出处
《分子植物育种》
CAS
北大核心
2023年第15期5019-5024,共6页
Molecular Plant Breeding
基金
贵州省科学技术基金项目(黔科合基础[2020]1Y100)
贵州省农科院青年基金项目(黔农科院青年基金[2018]85号)
贵州省水稻研究所青年基金项目(黔水稻所青年基金[2021]002号)共同资助。
关键词
水稻
剩余遗传变异
抽穗期
数量性状基因座
Rice
Residual genetic variation
Heading date
Quantitative trait locus
作者简介
通信作者:杨占烈,yzlgaas@163.com。
