摘要
为分析HMBOX1在非小细胞肺癌中的表达情况并探索其对肺癌细胞增殖和免疫逃逸能力的影响,探寻了HMBOX1在非小细胞肺癌中的生物学功能.采用免疫组化定量分析了肺癌组织和邻近正常组织中HM⁃BOX1的表达;采用细胞免疫荧光和western blot分析人正常支气管上皮样细胞系(16HBE)和人非小细胞肺癌细胞系(A549)中HMBOX1的表达;采用MTT法检测人自然杀伤细胞系(NK⁃92)对A549细胞的细胞毒作用;采用CCK⁃8法检测A549细胞的增殖能力.结果显示:HMBOX1在非小细胞肺癌组织中的表达显著低于癌旁正常组织(P<0.001),并且在A549细胞中的表达显著下降,低于16HBE细胞(P<0.001);与阴性对照组相比,A549细胞中的HMBOX1过表达后,细胞增殖能力受到抑制(P<0.001),并且NK⁃92细胞对A549细胞的细胞毒作用得到显著加强(P<0.01),A549细胞分泌的IFN⁃γ质量浓度显著下降(P<0.01).综上所述,HMBOX1能够抑制非小细胞肺癌细胞的增殖能力和免疫逃逸能力,可为非小细胞肺癌的免疫治疗提供新的作用靶点和治疗策略.
In order to analyze the expression of HMBOX1 in NSCLC and explore its effect on the proliferation and immune escape ability of NSCLC cells,the biological function of HMBOX1 in NSCLC was explored.The expression of HMBOX1 in lung cancer tissues and adjacent normal tissues was quantitatively analyzed by immunohistochemistry;the expression of HMBOX1 in human normal bronchial epithelioid cell line(16 HBE)and human non⁃small cell lung cancer cell line(A549)was analyzed by immunofluorescence and western blot;the cytotoxic effect of human natural killer cell line(NK⁃92)on A549 cells was detected by MTT assay,and the proliferation ability of A549 cells was detected by CCK⁃8 assay.The results show that the expression of HMBOX1 in NSCLC tissues was significantly lower than that in adjacent normal tissues(P<0.001),and the expression in A549 cells was significantly decreased,lower than that in 16 HBE cells(P<0.001);compared with the negative control group,the cell proliferation ability of A549 cells was inhibited after HMBOX1 overexpression(P<0.001),and the cytotoxic effect of NK⁃92 cells on A549 cells was significantly enhanced(P<0.01),and the concentration of IFN⁃γsecreted by A549 cells was significantly decreased(P<0.01).In summary,HMBOX1 is able to inhibit the proliferation ability and immune escape ability of non⁃small cell lung cancer cells and can provide new targets and therapeutic strategies for immunotherapy of non⁃small cell lung cancer.
作者
杨小珍
何丹
杨小桃
YANG Xiaozhen;HE Dan;YANG Xiaotao(Institute of Microbiology,Qujing Medical College,Qujing,Yunnan,China 655000;Department of Neurology,Qujing First People's Hospital,Qujing,Yunnan,China 655000;Dialysis Room,People's Hospital of Wuhua District,Kunming,Yunnan,China 650031)
出处
《昆明学院学报》
2023年第3期113-118,共6页
Journal of Kunming University
作者简介
杨小珍,男,云南富源人,曲靖医学高等专科学校实验师,研究方向为微生物与免疫学;通信作者:杨小桃,女,云南富源人,昆明市五华区人民医院住院医师,硕士,研究方向为临床肿瘤基础,E⁃mail:155179824@qq.com.
