摘要
该研究通过生长曲线和体外发酵实验对12株假小链双歧杆菌利用17种碳水化合物的能力进行了比较探究。从整体上看,假小链双歧杆菌能在葡萄糖、乳糖、低聚半乳糖培养基中达到较高的生长量(OD_(600)≥0.99),在蔗糖、低聚半乳糖培养基中达到高的生长速率(代时≤150 min)。研究发现12株假小链双歧杆菌对于碳水化合物利用具有较大的差异性,其中FJSNT36M3仅能利用5种碳水化合物,而3株菌(FHNBA14M1、FGZ16I1M1、FXJWS49M21)能利用10种以上。3株菌(FGSYC12M4、FGZ16I1M1、FFJND17M1)在9种碳源培养基中的生长速率(代时)和生长量(OD_(600))较高。12株假小链双歧杆菌编码的大多为降解植源性碳水化合物的糖苷水解酶,在碳水化合物利用中展现出的差异可能是因为降解和转运碳水化合物的酶具有差异性。人粪便体外发酵实验证明,假小链双歧杆菌对碳水化合物利用能力的不同与其占据肠道内丰度的潜力相关。
The utilization of carbohydrates by Bifidobacterium has always been a research focus,but the overall study on the utilization ability of carbohydrates by Bifidobacterium pseudocatenulatum is still lacking.In order to deepen the understanding of carbohydrate utilization in B.pseudocatenulatum,the growth curve and in vitro fermentation experiments were used to study the utilization ability of 12 strains of B.pseudocatenulatum to 17 carbohydrates.B.pseudocatenulatum can achieve high biomass in glucose,lactose,galactooligosaccharides(GOS)medium(OD_(600)≥0.99),and can reach a high growth rate(generation time≤150 min)in sucrose and GOS medium.The study also revealed significant inter-strain differences in carbohydrate utilization among the 12 strains,with FJSNT36M3 utilizing only five carbohydrates,while three strains(FHNBA14M1,FGZ16I1M1,FXJWS49M21)utilize more than 10 carbohydrates.On the generation time and OD_(600) indicator,three strains(FGSYC12M4,FGZ16I1M1,FFJND17M1)has a strong ability to utilize nine kinds of carbohydrates.Most of the glycoside hydrolases encoded by the 12 strains of B.pseudocatenulatum degrade plant-derived carbohydrates.The differences in carbohydrate utilization among strains may due to the differences in the enzymes that degrade and transport carbohydrates.In vitro fermentation experiments of human feces proves that the difference in carbohydrate utilization ability of B.pseudocatenulatum relates to its potential to occupy abundance in gut.
作者
李刘若兰
张程程
刘秉书
于雷雷
田丰伟
陈卫
翟齐啸
LI Liuruolan;ZHANG Chengcheng;LIU Bingshu;YU Leilei;TIAN Fengwei;CHEN Wei;ZHAI Qixiao(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2023年第14期94-102,共9页
Food and Fermentation Industries
基金
江苏省自然科学基金优秀青年基金(BK20200084)。
关键词
假小链双歧杆菌
碳水化合物利用
代时
OD600
体外发酵
Bifidobacterium pseudocatenulatum
carbohydrates utilization
generation time
OD 600
in vitro fermentation
作者简介
第一作者:李刘若兰,硕士研究生;通信作者:翟齐啸,教授,E-mail:zhaiqixiao@jiangnan.edu.cn。
