摘要
目的:探讨血清抗甲状腺球蛋白抗体(TGAB)水平与Graves病(GD)之间的关系。方法:首先分析101例GD患者血清TGAB、甲状腺过氧化物酶抗体(TPOAB)、促甲状腺素受体抗体(TRAB)、游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、三碘甲状腺原氨酸(T3)、甲状腺素(T4)、血清磷(P)和血清钙(Ca)的水平;分析TGAB与TPOAb、TRAB、FT3、FT4、T3、T4的相关性以及影响血清TGAB水平的危险因素;评估上述指标对GD患者的诊断效率;其次通过GEO数据库筛选GD差异表达基因,分析差异表达基因与甲状腺球蛋白(TG)水平的相关性及其相关调控miRNA。结果:血清TGAB水平与TPOAB、TRAB、FT3、FT4、T3和T4水平呈正相关关系(P<0.05)。与TGAB正常水平组患者相比,TPOAB、TRAB、FT3、FT4、T3和T4水平在高水平TGAB组患者血清中显著升高(P<0.05)。T3和TRAB是影响TGAB水平的危险因素。TGAB-TRAB-T3的联合诊断效率最高。GD的差异表达上调基因NCF1水平与TG水平呈正相关关系(r=0.5,P<0.05)。结论:血清TGAB-TRAB-T3联合检测明显提高GD的诊断效率;T3和TRAB水平升高的患者更容易出现TGAB水平升高;NCF1可能参与GD的发生发展,其水平可能受miR-431-5p的调控。
Objective:To explore the correlation between the serum level of anti-thyroglobulin antibody(TGAB)and Graves’disease(GD).Methods:The levels of serum anti-thyroglobulin antibody(TGAB),thyroid peroxidase antibody(TPOAB),thyroid stimulating hormone receptor antibody(TRAB),free triiodothyronine(FT3),free thyroxine(FT4),triiodothyronine(T3),thyroid hormone(T4),serum phosphorus(P)and serum calcium(Ca)in 101 patients with GD in this study and the correlation between TGAB and TPOAB,TRAB,FT3,FT4,T3 as well as T4 were first analyzed.Meanwhile,the risk factors affecting serum TGAB levels were analyzed,and the diagnostic efficiency of the above indicators for GD patients was evaluated.And then differentially expressed genes(DEGs)in GD were screened from the GEO database to analyze the correlation between differentially expressed genes and thyroglobulin(TG)levels and their related regulatory miRNAs.Results:The serum levels of TGAB were positively correlated with the levels of TPOAB,TRAB,FT3,FT4,T3,T4,P and Ca in GD patients(P<0.05).The serum levels of TPOAB,TRAB,FT3,FT4,T3 and T4 in patients with high TGAB levels were significantly higher than those in patients with normal TGAB levels(P<0.05).T3 and TRAB were the risk factors affecting TGAB levels.The combined diagnostic efficiency of TGAB-TRAB-T3 was the best.The NCF1 levels of differentially expressed up-regulated genes in GD were positively correlated with TG levels(r=0.5,P<0.05).Conclusion:The combined detection of serum TGAB-TRAB-T3 significantly improves the diagnostic efficiency of GD,and patients with elevated levels of T3 and TRAB are more likely to experience elevated levels of TGAB.NCF1 may be involved in the occurrence and development of GD,and its level may be regulated by miR-431-5p.
作者
肖毅
林发全
刘奕
邱卓妍
李成
梁裕玮
梁晓君
凌维璟
曾麒燕
Xiao Yi;Lin Faquan;Liu Yi;Qiu Zhuoyan;Li Cheng;Liang Yuwei;Liang Xiaojun;Ling Weijing;Zeng Qiyan(Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,China;Department of Clinical Laboratory,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;Guangxi Colleges and Universities Key Laboratory of Biological Molecular Medicine Research,Nanning 530021,China)
出处
《广西医科大学学报》
CAS
2023年第4期638-644,共7页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.82060387)。
作者简介
通信作者:曾麒燕,E-mail:zengnn6@hotmail.com。
