摘要
目的响应面法优化大花红景天总酚提取工艺,分析大花红景天总酚含量与抗氧化活性及其相关性,为大花红景天资源开发与适宜采收时间提供科学依据。方法在单因素试验基础上,利用Box-Behnken响应面法优化大花红景天总酚提取工艺,并测定大花红景天提取液对DPPH·和ABTS^(+)·清除能力的影响。结果大花红景天总酚最佳提取工艺:乙醇浓度为50%、提取温度为50℃、提取时间为40 min,在此条件下提取率可达为12.22 mg/g;野生样品总酚含量和抗氧化活性均显著高于人工栽培品,人工栽培品总酚含量以9月20日最高。大花红景天提取液清除DPPH·能力强于清除ABTS^(+)·,总酚含量与DPPH·和ABTS^(+)·的半数抑制浓度(IC_(50))值呈显著负相关(r<0,P<0.01)。结论响应面优化法试验理论值与实测值较一致,可用于大花红景天总酚提取分析和预测;大花红景天具有较强体外抗氧化活性,并与总酚含量呈显著正相关;大花红景天人工栽培品适宜采收期为9月下旬。
Objective To optimize the extraction process of total phenol from Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba by response surface method,and to analyze the content of total phenol and antioxidant activity of Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba and their correlation,so as to provide scientific basis for resource exploitation and appropriate harvesting time of Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba.Methods On the basis of single factor test,Box-Behnken response surface method was used to optimize the extraction process of total phenol from Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba.The effects of Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba extract on the scavenging ability of DPPH·and ABTS^(+)·were determined.Results The optimum extraction conditions of Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba were as follows:ethanol concentration of 50%,extraction temperature of 50℃,and extraction time of 40 min.Under these conditions,the extraction rate could reach 12.22 mg/g.The total phenol content and antioxidant activity of wild samples were significantly higher than those of artificial cultivars,and the total phenol content of artificial cultivars was the highest on September 20.The DPPH·scavenging ability of Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba extract was stronger than ABTS^(+)·scavenging ability.The total phenol content was negatively correlated with median inhibitory concentration(IC_(50))values of DPPH·and ABTS^(+)·(r<0,P<0.01).Conclusion The theoretical values of the response surface optimization method are consistent with the measured values,which can be used to extract and predict the total phenol of Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba.Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba have strong antioxidant activity in vitro and is positively correlated with total phenol content.The suitable harvest time of Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba artificial cultivars is late September.
作者
孙羽珂
谢叶林
唐小慧
赵文吉
何正军
贾国夫
黎剑
陈宇航
SUN Yuke;XIE Yelin;TANG Xiaohui;ZHAO Wenji;HE Zhengjun;JIA Guofu;LI Jian;CHEN Yuhang(School of Pharmacy,Chengdu Medical College,Sichuan Province,Chengdu 610500,China;Research Center of Engineering Technology of Ecological Remediation of Alpine Grassland in Qinghai-Tibet Plateau,Sichuan Academy of Grassland Sciences,Sichuan Province,Chengdu 611731,China;Institute of Agro-Products Processing,Sichuan Academy of Agricultural Sciences,Sichuan Province,Chengdu 610066,China)
出处
《中国医药导报》
CAS
2023年第18期30-34,共5页
China Medical Herald
基金
四川省科技计划省院省校合作项目(2019YFSY-0010)
四川省级科研院所基本科研业务费项目(2022005)
成都医学院省级大学生创新创业训练计划项目(S202113705056)。
关键词
大花红景天
总酚提取
响应面优化
抗氧化活性
采收期
Rhodiola crenulata(HooK.f.et.Thoms)H.Ohba
Total phenols extraction
Response surface optimization
Antioxidant activity
Harvest time
作者简介
通讯作者:陈宇航(1983-),男,博士,副教授,研究方向:中药资源学;通讯作者:赵文吉(1986-),男,硕士,研究方向:高原药用植物资源培育与开发。
