摘要
目的 研究纳米钙补肾中药通过MAPK/ERK通路对髋关节骨质疏松大鼠骨密度的影响。方法 将30只SD大鼠随机分成对照组、模型组及治疗组,每组10只。对治疗组和模型组大鼠构建髋关节骨质疏松模型。对照组大鼠采用常规喂养8周。测定三组大鼠髋关节骨密度、血清磷及血清钙含量,检测髋关节骨生物力学,通过髋关节骨HE染色及骨组织形态剂量学观察参数,运用蛋白印迹法检测Ras、ERK、PKC、PKAG蛋白表达水平。结果 与对照组相比,模型组和治疗组大鼠骨密度、血清钙以及Tb·Ar、Tb·Th、Tb·N指标含量明显降低,血清磷、Tb·Sp指标含量明显升高(P<0.05);与模型组相比,治疗组大鼠骨密度、血清钙以及Tb·Ar、Tb·Th、Tb·N指标含量明显升高,血清磷、Tb·Sp指标含量明显降低(P<0.05)。模型组和治疗组大鼠的最大载荷、抗压强度、轴向刚度及水平剪切刚度低于对照组,其中治疗组高于模型组(P<0.05)。模型组和治疗组大鼠的Ras、ERK、PKC、PKAG蛋白表达水平高于对照组,其中治疗组低于模型组(P<0.05)。结论 纳米钙补肾中药可通过调节MAPK/ERK通路,下调Ras、ERK、PKC、PKAG蛋白的表达水平进而提高髋关节骨质疏松大鼠的骨密度、骨小梁数量、骨小梁厚度、骨小梁面积百分数,降低骨小梁分离度。
Objective To study the effect of nano calcium kidney tonifying traditional Chinese medicine on bone mineral density in hip osteoporotic rats through MAPK/ERK pathway.Methods Thirty SD rats were randomly divided into control group,model group and treatment group,with 10 rats in each group.The osteoporotic model of hip joint was established in the treatment group and the model group.The rats in the control group were routinely fed for 8 weeks.The bone mineral density of hip joint,the content of serum phosphorus and serum calcium,the bone biomechanics of hip joint,the HE staining of hip joint and the dosimetric observation parameters of bone histomorphology were measured,and the protein expression levels of RAS,ERK,PKC and pkag were detected by Western blot.Results Compared with the control group,the BMD,serum calcium and the contents of Tb·Ar,Tb·Th and Tb·N indexes in the model group and the treatment group were significantly decreased,while the contents of serum phosphorus and Tb·Sp indexes were significantly increased(P<0.05).Compared with the model group,the BMD,serum calcium and the contents of Tb·Ar,Tb·Th and Tb·N indexes in the treatment group were significantly increased,and the contents of serum phosphorus and Tb·Sp indexes were significantly decreased(P<0.05).The maximum load,compressive strength,axial stiffness and horizontal shear stiffness of rats in the model group and the treatment group were lower than those in the control group,and those in the treatment group were higher than those in the model group(P<0.05).The expression levels of RAS,ERK,PKC and pkag in model group and treatment group were higher than those in control group,and those in treatment group were lower than those in model group(P<0.05).Conclusion By regulating MAPK/ERK pathway,nano calcium kidney tonifying traditional Chinese medicine can down regulate the expression levels of RAS,ERK,PKC and pkag proteins,improve the bone mineral density,number of trabecular bone,thickness and area percentage of trabecular bone in the rat model of hip osteoporosis,and reduce the separation degree of trabecular bone.
作者
赖子云
吴伯文
慕洪旭
文天一
蔡书勤
喻琴
LAI Ziyun;WU Bowen;MU Hongxu;WEN Tianyi;CAI Shuqin;YU Qin(Pingkuang General Hospital,Pingxiang 337000,China;Pingxiang People’s Hospital,Pingxiang 337099,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2023年第6期792-795,801,共5页
Chinese Journal of Osteoporosis
基金
江西省卫生健康委科技计划(202212085)。
关键词
MAPK/ERK通路
纳米钙补肾中药
骨质疏松症
骨密度
MAPK/ERK pathway
nanometer calcium combined with tonifying kidney herbs
osteoporosis
bone density
作者简介
通信作者:喻琴,Email:laiziyunui@163.com。
