摘要
The application of fungal biotechnology for nutrient value addition and improvement of agro-industrial wastes is critical in the development of protein-rich feed for livestock.This study evaluated conventional methods for protein enrichment of cassava peels by solid substrate fermentation using mono-and co-cultures of Aspergillus niger and Trichoderma viride.Sterile and non-sterile cassava peels inoculated with mono-cultures of A.niger or T.viride and co-cultures of A.niger and T.viride were spontaneously fermented for 21 days at 24℃.Fermented substrates were harvested at 0,7,14,and 21 days intervals,then oven-dried at 60℃,milled in a blender and further assayed for ash,carbohydrate,crude fibre,fat,and protein contents.Results showed that percentage increase in protein contents after 21 days incubation of sterile cassava peels were 71.02%(for mono-culture of A.niger)and 71.64%(for mono-culture of T.viride);co-culture of A.niger and T.viride yielded a 129.00%increase in protein.Enhanced percentage increase in protein content of the non-sterile cassava peels was 126.80%(for mono-culture of A.niger)and 168.34%(for mono-culture of T.viride)in contrast with 63.47%obtained for the co-culture of A.niger and T.viride in non-sterile cassava peels,which was attributable to possible antibiosis due to microbial competition.Ash content significantly(p≤0.05)increased for 21 days while carbohydrate,crude fibre and fat contents in both sterile and non-sterile cassava peels decreased over the same period.These findings underscore the fact that protein content of cassava peels can be significantly(p≤0.05)enriched through fungal biotechnology to produce value-added feed supplement for livestock.The practical implications of these findings are discussed.
作者简介
Corresponding Author:Yafetto L,e-mail:lyafetto@ucc.edu.gh。
