摘要
醇脱氢酶(alcohol dehydrogenase,ADH)是生物体内主要短链醇代谢的关键酶,在物质代谢和能量转化过程中发挥着重要作用。本文从玉米大斑病菌(Setosphaeria turcica)基因组的KEGG数据库中获得了22个ADH基因家族成员(StADH1-StADH 22),它们散布在12条scaffold上,长232~495 aa,理论等电点5.65~9.22,81.8%家族成员呈酸性,72.7%为亲水蛋白,有4个家族成员定位于线粒体,2个定位于过氧化物酶体,其余16个家族成员均定位于细胞质。系统发育分析结果表明,有11个玉米大斑病菌ADH家族蛋白与酵母ADH(YADH1-7)亲缘关系较近,含有相似的保守基序和结构域,其他11个玉米大斑病菌ADH家族蛋白与酵母ADH亲缘关系较远,含有不同的保守基序和结构域。玉米大斑病菌侵染玉米叶片的转录组分析结果表明,仅有4个ADH基因(StADH 1、StADH 4、StADH 6和StADH 12)参与病菌侵染过程,其中StADH 1、StADH 4和StADH 12主要在病菌侵染后期发挥作用,而StADH1还可能与病菌的致病专化性有关。
Alcohol dehydrogenase(ADH)is a major enzyme of short chain alcohol metabolism in all organisms and plays an important role in the process of substance metabolism and energy conversion.Utilizing the KEGG database of Setosphaeria turcica genome,22 members of the ADH gene family(StADH1-StADH 22)of Setosphaeria turcica were obtained in this paper.The ADH genes were distributed over 12 scaffolds with 232-495 aa in length,the theoretical isoelectric point of 5.65-9.22,81.8%acidic family members and 72.7%hydrophilic proteins.Four family members were located in mitochondria,2 members in peroxisome,and the other 16 family members were located in cytoplasm.Phylogenetic analysis results show that there are 11 Setosphaeria turcica ADH family proteins closely related with yeast ADH gens(YADH1-7)which have the similar conservative motifs and structure domains,and the other 11 StADH family proteins are far from yeast ADH and contain different conservative motifs and structure domains.Through the analysis of Setosphaeria turcica-Zea mays gene expression transcriptome,there are four StADH family genes StADH1,StADH4,StADH 6 and StADH 12 participate in the pathogen infection process,and StADH1,StADH 4 and StADH 12 play very important roles in the later period of infection.Furthermore,StADH 1 might be related to the pathogenicity specificity of the pathogen.
作者
张淑红
张运峰
武秋颖
高凤菊
李亚子
纪景欣
许可
范永山
ZHANG Shuhong;ZHANG Yunfeng;WU Qiuying;GAO Fengju;LI Yazi;JI Jingxin;XU Ke;FAN Yongshan(Department of Life Sciences,Tangshan Normal University,Tangshan Key Laboratory of Agricultural Pathogenic Fungi and Toxins,Tangshan 063000,Hebei,China)
出处
《浙江农业学报》
CSCD
北大核心
2023年第5期1108-1115,共8页
Acta Agriculturae Zhejiangensis
基金
唐山市人才项目(A202007009)
唐山师范学院科研项目(2021B30,2021B34)
唐山师范学院重点培育项目(ZDPY06,ZDPY08)。
作者简介
张淑红(1978—),女,河北赞皇人,硕士,副教授,主要从事微生物遗传研究。E-mail:zhangshuhong@tstc.edu.cn;通信作者:范永山,E-mail:fanyongshan@126.com。
