摘要
目的:利用网络药理学方法探讨人参四物汤治疗冠状动脉粥样硬化性心脏病(简称冠心病)的作用机制。方法:通过中药系统药理学数据库与分析平台(TCMSP)网络数据库对人参四物汤的有效成分及作用靶点进行挖掘和筛选,利用Uniprot蛋白质数据库寻找蛋白质靶点所对应的人类基因,同时以“coronary heart disease”为关键词挖掘Gene Cards、TTD、DRUGBANK数据库中冠心病相关作用靶点,利用微生信在线数据分析作图网站制作韦恩图,并将所得交集靶点导入Cytoscape 3.7.2,构建“药物-有效成分-靶点-疾病”网络。基于STRING数据库,构建蛋白质-蛋白质相互作用网络模型;再利用Bisogenet进行拓扑分析,筛选出关键靶点;最后利用Metascape数据库对药物-疾病交集靶点进行生物功能和通路的富集分析。结果:人参四物汤治疗冠心病的核心活性成分为山柰酚、豆甾醇、β-谷甾醇等。核心靶点为核磷脂素1(NPM1)、异质性胞核核糖核蛋白U(HNRNPU)、热休克蛋白A5[Heat Shock Protein Family A(Hsp70)Member 5,HSPA5]、热休克蛋白A8[Heat Shock Protein Family A(Hsp70)Member 8,HSPA8]、人真核翻译延伸因子1a1(EEF1A1)、异质性胞核核糖核蛋白P2(FUS)、核糖体蛋白S27A(RPS27A)、反式激活应答DNA结合蛋白(TARDBP)等。关键通路为脂质与动脉粥样硬化、血流剪切力与动脉粥样硬化、白细胞介素-17(Interleukin-17,IL-17)信号通路等。结论:人参四物汤通过多成分、多靶点、多通路治疗冠心病,为临床冠心病的治疗提供了新的思路。
Objective:Using the network pharmacology method to explore the mechanism of the Renshen Siwu decoction(人参四物汤)in the treatment of coronary atherosclerotic heart disease.Methods:The effective components and targets of the Renshen Siwu decoction were excavated and screened through TCMSP network database,and the human genes corresponding to protein targets were found by UniProt protein database.At the same time,taking coronary heart disease as the keyword,the CHD related targets in GeneCards,TTD and DRUGBANK databases were mined,Venn picture was made by Weishengxin(微生信)online data analysis and mapping website,and the obtained intersection targets were imported into Cytoscape 3.7.2 to build a drug-effective component-target-disease network.After that,the protein-protein interaction network model was constructed based on STRING database,and then Bisogenet was used for topology analysis to screen out key targets.Finally,the Metascape database was used to enrich and analyze the biological functions and pathways of drug disease intersection targets.Results:The core active components of the Renshen Siwu decoction in the treatment of coronary heart disease were kaempferol,stigmasterol,beta-sitosterol,etc..The core targets are NPM1,HNRNPU,HSPA5,HSPA8,EEF1A1,FUS,RPS27A,TARDBP,etc..The key pathways are lipid and atherosclerosis,fluid shear stress and atherosclerosis,IL-17 signaling pathway,etc..Conclusion:The Renshen Siwu decoction provides a multi-component,multi-target,multi-pathway method for the treatment of coronary heart disease.
出处
《中医临床研究》
2023年第8期1-9,共9页
Clinical Journal Of Chinese Medicine
基金
国家科技重大专项(2017ZX09301003)。
作者简介
潘月华,女,2020级硕士研究生,研究方向为中西医结合治疗心血管疾病;通讯作者:张世亮,医学博士,教授,博士研究生导师,主任医师,研究方向为中西医结合治疗心血管疾病。
